摘要
激素敏感脂肪酶（ＨＳＬ）是动物体脂肪分解的关键酶。采用ＰＣＲ－ＳＳＣＰ法研究猪ＨＳＬ基 因的启动子区、部分５’一端转录非翻译区、第７外显子区、第８外显子区和３’一端部分转录非翻 译区域ＤＮＡ多态性。结果在对应于ＨＳＬ基因第８外显子区域发现１ＤＮＡ片段的ＳＳＣＰ，表现 出３种基因型ＭＭ、ＭＮ和ＮＮ，脂肪型猪种梅山猪和通城猪含有更多的等位基因Ｍ（频率分别 为０．６９０和０．７４０），而瘦肉型猪种大白猪和长白猪则含有更多的等位基因Ｎ（频率分别为０．８４７ 和 ０．８４５）；该 ＤＮＡ片段经测序分析，发现位于基因ｃＤＮＡ第 ２ ２４９和 ２ ２８０处发生碱基序列的 改变（均为 Ａ→Ｇ），使天冬酰胺变为天冬氨酸（Ａｓｎ→Ａｓｐ）和谷氨酸变为甘氨酸（Ｇｌｕ→Ｃｌｙ）。
Hormone sensitive lipase (HSL) is a key enzyme in fat metabolism. The polymorphism of the pig HSL gene is studied in this paper. The SSCP (Single Strand Conformational Polymorphism) is found in the HSL gene Exon 8. It shows three genotypes (MM,MN,NN). Fat-type pig breeds of Meishan and Tongcheng pigs have more allele M with frequencies of 0.690M and 0.740M respectively; however, lean-type pig breeds of Landrace and the Large White Pigs have more allele N with frequencies of 0.847N and 0.845N, respectively. PCR fragments representing HSL genotypes MM and NN are sequenced. Two A-G transitions are detected, and it resulted in corresponding changes of amino acid (Asn-Asp and Glu-Gly) in the HSL protein, respectively. The HSL gene can be considered as a candidate gene for fatness in pigs.
基金
国际科学基金
