大麦6H染色体特异性标记的筛选和鉴定

The Chromosome-specific PCR Marker's Screening and Identication of Barley 6H Chromosome

从大麦、小麦和小麦－大麦６Ｈ染色体附加系ＲＡＰＤ分析筛选出对６Ｈ染色体特异的２个 ＲＡＰＤ标记，转换为特异性ＰＣＲ标记，利用该标记对不同植物材料进行ＰＣＲ扩增鉴定。表明凡 含有大麦６Ｈ染色体的材料（Ｂｅｔｚｅｓ、Ｉｇｒｉ、ＣＳ ６Ｈ附加系）均能扩增出特异带；而不含６Ｈ染色体的 材料，包括小麦、黑麦、长穗偃麦草、中间偃麦草、簇毛麦以及含有其他大麦染色体的小麦附加系 均没有扩增出特异带。可见，２对ＰＣＲ引物具有大麦６Ｈ染色体特异性，表明用ＲＡＰＤ标记转换 为ＰＣＲ标记鉴定大麦特定染色体的方法可行。并通过Ｓｏｕｔｈｅｒｎ杂交证明这２个克隆片段分属 于大麦基因组特异的多拷贝重复序列和小麦与大麦基因组共有的寡拷贝序列。

Two barley 6H chromosome specific RAPD markers were obtained by screening DNA of barley Hordeum vulgare (Betzes) and wheat-barley 6H addition line with 200 primers, then the RAPD markers were changed into specific PCR markers. Checking different plant materials by the PCR markers, it revealed that there was a specific band in those materials containing 6H chromosome such as Betzes, Igri, CS6H,and there was no specific band if the material did not contain 6H chromosome, such as Triticum aestivum, Secale cereale, Agropyron intermedium, Haynaldia villosa, Thinopyrum elongatum. Therefore, those PCR markers specific to chromosome 6H of barley are established. Southern hybridization indicated that the two cloned DNA fragments belong to barley genomic specific high-copy repeat sequence and low-copy sequence in wheat and barley genomes respectively.