红莲型水稻与细胞质雄性不育相关mtDNA片段的分离及序列测定

ISOLATION AND SEQUENCE ANALYSIS OF A MITOCHONDRIAL DNA FRAGMENT ASSOCIATED WITH CMS IN HONG LIAN TYPE RICE

应用AFLP分析技术,比较了红莲型水稻雄性不育系和保持系线粒体DNA,找到一不育系特异条带TA1。以该条带DNA为探针与不育系、保持系以及F_1杂种线粒体总RNA杂交,结果显示该片段黄化苗期三者均转录一个RNA分子,但转录产物分子量各不相同,说明三者该片段阅读框架不同。经测序,该片段长202bp,序列内含密码子ATG、ATT、AGA、AGG以及正向重复序列5’TGTAC3’、5’ATTATTTT3’和倒转重复序列5’GGGAAACA3’。上述特点表明该片段可能是某一蛋白编码序列,并可能与红莲型水稻雄性不育性状形成有关。

A CMS Line specific mtDNA band TA1 was isolated by comparing the mtDNAs of CMS tine and maintainer line using AFLP technique. The Northern blot analysis of the TA1 mtDNA fragment show that it transcripts only one RNA molecular each in CMS line, maintain line and the first filial generation, but the RNA moleculars are different among each other. By sequencing, the fragment is found to be 202 base pairs in length. It contains code ATG, ATT, AGA and AGG, two small repeated sequences 5'TGTAC3' and 5'ATTATTTT3' and one small inverted-repeated sequence 5'GGGAAACA3'. The results imply that the mtDNA fragment may be a coding region and may be relative to CMS of Hong Lian type rice.