血管紧张素转换酶基因I/D多态性与高血压病患者血清、尿β_2微球蛋白及尿白蛋白的关系

Serum and urinary levels of β_2-Microglobulin-excretion and urinary albumin-excretion and ACE gene I /D polymorphism associated with essential hypertension

目的探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性与高血压病患者血尿β2微球蛋白(β2-MG)及尿白蛋白的关系。方法应用多聚酶链反应(PCR)方法对177例原发性高血压病患者和60例正常对照组ACE基因I/D多态性进行测定及用放射免疫分析法测定血清、尿β2微球蛋白及尿白蛋白。结果①高血压病组患者的血清及尿β2微球蛋白,尿白蛋白明显高于对照组(P<0.01),以尿蛋白升高更明显(P<0.001);②高血压病患者各ACE基因型血清β2微球蛋白增高以DD、ID基因型明显(P<0.01,P<0.05),而尿白蛋白以DD型明显(P<0.05)。结论 ACE基因I/D多态性与高血压病患者的血清、尿β2微球蛋白及尿白蛋白明显相关。D等位基因与高血压病早期肾损害有关。血清、尿β2微球蛋白及尿白蛋白的检测是反应高血压肾损害早期检测指标。

Objective To observe relationship between Serum and urinary levels of β2-Microglobulin-excretion and urinary albu- min- excretion and ACE gene I/D polymorphism on patients with essential hypertension（EH）. Methods The polymerase chain reaction（PCR） and ultraviolet methods were used to determine the ACE gene I/D polymorphism and the radiommunoassay to measure theβ2-Microglobulin-excretion and urinary albumin-excretion in the study of 177 essential hypertension patients and 60 control subjects. （age, sex matched）. Results （1）In all patients with EH, the levels of serum and urinaryβ2-Microglobulin-excretion and urina- ry albumin- excretion present increase compared with control subjects. The levels of urinary albumin- excretion is the highest（ P 〈 0. 001 ）. （2）All patients with EH carried ACE gene ID and DD genotype the levels of serum β2-microglobulin-excretion was signifi- cantly higher than those of II genotyoe＇s （ P 〈 0. 01, P 〈 0.05 ）. Conclusion A significant relationship has been found between the polymorphism of the ACE gene and the levels of serum and urinary β2-microglobulin-excretion and urinary albumin-excretion. The D allele seems to predispose to early renal complication.