佛手饮料废渣中分离柠檬苦素的研究

Extraction of limonin from residue of berganot juice

采用Sephadex LH-20凝胶柱色谱分离纯化了佛手饮料废渣中的柠檬苦素,分离条件:检测器波长为207 nm;柱体积为112.5 mL,分离柱内径为1.5 cm;流动相为甲醇,流速为0.5 mL/min.用核磁共振法对分离产物进行了结构鉴定.通过高效液相色谱法测定了佛手饮料废渣中柠檬苦素的含量,测定条件:采用美国AGT Venusil XBP(L)ODS C18柱(250 mm×4.6 mm,10μm);流动相V(乙腈)∶V(水)=45∶55,流速为0.8mL/min;柱温为25℃;检测波长为207 nm.结果显示,佛手饮料废渣中柠檬苦素含量为1.73 mg/g,相对标准偏差为1.1%.

Limonin and the wavelength in waste residue of of detector was set bergamot was separated by Sephadex to 207 nm ; column volume was 112.5 LH-20 column chromatography, mL, the diameter of column was 1.5 cm ; with methanol as 100% mobile phase and flow rate flow rate was 0.5 mL/min, NMR was used to structural identification after the separation. HPLC was used to determine the quantity of limonin. Chromatographic conditions： American AGT Venusil XBP （ L） ODS C18 （250 mm ×4.6 mm, 10 μm） column with acetonitrile-water, 45：55 （V/V） as mobile phase and flow rate 0.8 mL/min. The column temperature was controlled at 25 ℃ and the wavelength of detector was set to 207 nm. The results showed that the RSD was 1.1% and the limonin content in waste residue of bergamot under those conditions reached 1.73 mg/g.