丛枝菌根真菌对矿区修复系统固碳的作用

Role of arbuscular mycorrhizal fungi in reclaimed mine soil system carbon sequestration

采用煤矸石为主体基质添加活性污泥,同时接种丛枝菌根真菌(AMF)于新栽植的黑麦草,进行为期16个月的现场种植试验.对比分析了黑麦草根部AMF的侵染状况,及其对土壤固碳的促进作用.结果表明:接种摩西球囊霉菌(Glomus mosseae,简记为G.m)和添加活性污泥量30%基质的菌根侵染率最高值为66.67%;接种处理的土壤有机碳(SOC)、易提取球囊霉素(EEG)、总球囊霉素(TG)含量和植物生物量远高于未接种处理,添加活性污泥量30%基质的增值最高.土壤有机碳及植物生物量与菌根侵染率、球囊霉素相关土壤蛋白(GRSP)间的相关性分析表明,AMF所分泌的代谢产物总球囊霉素TG与矿区复垦土壤SOC呈现正相关性(相关系数为0.699).AMF及其GRSP对于矿区复垦土壤固碳的贡献是直接有效的.

A field experiment is carried out to evaluate the carbon sequestration capacity in reclaimed mine soil by adding sludge to the coal gangue substrate and inoculated arbuscular my-corrhizal fungi （AMF） to the rye grass. The inoculation situations of AMF to the roots of rye grass is compared, and the promotion to the carbon sequestration in soil is also investigated. The results show that mycorrhizal colonization rate of treatment with 30% weight sludge addition, inoculated with Glornus mosseae （G. m） is the highest, 66.67%. Soil organic carbon （SOC）, easily extractable g]omalin （EEG） and total glomalin （TG） and plants biomass of treatments inoculated with G. m were higher than those non-inoculated, and the increase of treatment with 30% weight sludge addition inoculated with G. m was the highest. Relativity of TG and SOC is 0. 699. Therefore, AMF and glomalin-related soil protein（GRSP） have direct and effective contribution to carbon sequestration in reclaimed mine soil.