一株抗铜木霉对含铜废水的修复潜力研究

Bioremediation potential of a copper-resistant Trichoderma strain for copper-containing wastewater

基于真菌作为含铜废水生物修复剂的优点,探讨了一株里氏木霉FS10-C对含铜废水的修复潜力.采用摇瓶试验研究了不同Cu2+含量溶液下木霉FS10-C的生长发育和铜积累率;扫描电子显微镜(SEM)和X射线光散射能谱(EDX)观察成熟FS10-C细胞吸附Cu2+后形态和各元素变化;同时进行了该株木霉的生物安全性初步评价.结果表明:FS10-C孢子在pH为(4.0±0.2)～(5.6±0.2),Cu2+的质量浓度在不大于600mg/L的溶液中均可生长发育,且在质量浓度为100,200和400mg/L的Cu2+溶液中其铜积累率分别达50.6%,30.3%和4.7%.SEM显示,在质量浓度为100mg/L的Cu2+溶液中,成熟FS10-C细胞形态无改变,400mg/L时菌体结构仍完整;EDX元素分析显示,在质量浓度为100,200和400mg/L的Cu2+溶液中,FS10-C细胞铜的平均质量分数分别为18.50%和30.40%,且菌丝大于孢子.生物安全性初步评价表明FS10-C及代谢产物对人畜均无害.里氏木霉FS10-C是一株颇具研究潜力的含铜废水修复菌.

Based on the advantages of fungi as a bioremediation agent for copper-containing wastewater, bioremediation potential of Trichoderma reesei FS10-C for copper-containing wastewater was investigated. Flask tests were conducted on analyzing the development, growth and accumulation rate of FS10-C under copper stress in aqueous solutions. Scanning e- lectron microscope （SEM） and energy-dispersive X-ray analysis （EDX） were used to observe the morphology and element variations of FSI0-C, and bio-safety assessment of the strain was also performed. The results show that the spore of FS10-C can grow and develop in aqueous solutions with pH of （4.0±0. 2）- （5.6±0. 2） and Cu（ Ⅱ ） concentration below 600 mg/L.Copper accumulation rates of FS10-C under 100, 200 and 400 mg/L Cu（ Ⅱ ） concentrations were 50.60/oo, 30.3% and 4.70%, respectively. The SEM showed no morphological variation in FS10-C at 100 mg/L Cu（Ⅱ） and mostly complete cell structure at 400 mg/L Cu（Ⅱ）. EDX results showed that weight percentages of copper in FS10-C were 18. 50% and 30.40% at 100 and 400 mg/L Cu（Ⅱ）, respectively, and biosorption ability of mycelium was higher than that of spore. Bio-safety assessment indicated that both FS10-C and its metabolites were harmless to human and animal health. The results suggest that Trichoderma reesei FS10-C may be a good bioremediation agent for copper-containing wastewater.