僵蚕对哮喘豚鼠血清IL-4和IFN-γ的影响

Effects of Bombyx Batryticatus on IL-4 and IFN-γ of asthmatic model in guinea pigs

目的:探索僵蚕对哮喘模型豚鼠血清中IL-4和IFN-γ水平的影响。方法:用卵白蛋白致敏法制备哮喘豚鼠模型并以卵白蛋白激发哮喘发作后,予僵蚕或地塞米松治疗。采用ELISA法检测哮喘豚鼠血清中IL-4和IFN-γ的水平。结果:与模型组比较,僵蚕高、低剂量水提液及地塞米松均能降低哮喘模型豚鼠血清中IL-4水平,具有显著差异性(P<0.01);并能提高IFN-γ水平(P<0.05)。结论:僵蚕水提液可能通过调节机体IL-4和IFN-γ的水平,达到调节Th1/Th2平衡,有效控制哮喘发作。

Objective： To study the effect of Bombyx Batryticatus on IL-4 and IFN-T in blood serum of asthmatic model in guinea pigs. Methods： Asthmatic models of guinea pigs were constructed by immunization and challenged with nebulized OVA, then they were treated with Bombyx Batrytieatus or dexamethasone.The levels of IL-4 and IFN-γ in of guinea pigs were determined by using ELISA. Results： In the asthmatic model group, the levels of IL-4 had significant differences compared with those of high Bombyx Batrytieatus group, low Bombyx Batryticatus group and dexamethasone group （P〈0.01）. The levels of IFN-γ, were increased in high Bombyx Batryticatus group, low Bombyx Batryticatus group and dexamethasone group （P〈0.05）. Conclusion： Bombyx Batryticatus may regulate the Thl/Th2 balance by influence the balance ofiL-4 and IFN-7, which play an important role in treating asthma.