血管内皮生长因子受体与双绒毛膜双胎生长不一致的相关性

A study of the association between vascular endothelial growth factor receptors and dichorionic twins discordance

目的探讨血管内皮生长因子受体-1(Flt-1)、可溶性血管内皮生长因子受体-1(sFlt-1)与双绒毛膜双胎生长不一致的关系。方法收集2009年10月至2011年1月在中山大学附属第一医院产科分娩的双绒毛膜双胎40例为研究对象,包括17例生长不一致性双胎及23例生长一致性双胎,应用酶联免疫吸附(ELISA)法检测脐血清中sFlt-1;免疫组织化学法检测胎盘组织中Flt-1蛋白的表达量。结果生长不一致性双胎中小胎脐血清中sFlt-1[(3366.40±479.36)ng/L]明显高于大胎[(2031.80±373.17)ng/L]及对照组[小胎和大胎分别为(1892.08±631.70)ng/L和(1903.00±580.16)ng/L](P<0.05);生长不一致性双胎中小胎胎盘中Flt-1蛋白表达量明显低于大胎及对照组(P<0.05)。结论 Flt-1、sFlt-1表达异常与双绒毛膜双胎生长不一致密切相关。

Objective To study the levels of sFlt-1 in umbilical vein serum and the expressions of Flt-1 protein on placenta of twins discordance, providing more information for discussing the relationship between sFh-1, Flt-1 and twins discordance. Methods Collected 40 women with dichorionic twins, including 17 twins discordance and 23 twins concordance. The cases came from Department of Gynaecology and Obstetrics of the First Affiliated Hospital of Sun Yat-sen University. Enzyme linked immunosorbent assay technique was used to measure the serum level of sFlt-1. Fh-1 content and distribution in the placenta tissue was measured by immunohistochemistry. Results The results showed that the serum sFlt-1 level of the smaller fetus in twins discordance was relatively higher than the larger fetus and those of control group （ P 〈 0. 05）. The contents of Fh-1 protein in placenta of the smaller fetus in twins discordance were relatively lower than the larger fetus and those of control group （P 〈 0.05）. Conclusion The abnormal expression between sFlt-1 and Flt-1 was closely related to dichorionic twins discordance.