乙型肝炎病毒大蛋白检测的临床意义

Study on the clinical signification of serum epatitis B virus large surface protein levels

目的分析乙型肝炎病毒大蛋白(L HBS)与HBV-DNA的相关性,探讨L HBS在乙型肝炎临床检测中的意义。方法以ELISA法检测样品L HBS和定量PCR法检测HBV-DNA。结果218例HBsAg阳性和HBeAg阳性的血清样本中,L HBS阳性率为95.41%,HBV-DNA的阳性率为92.66%,两者阳性率差异统计学意义(χ2=81.36,P<0.001);120例HBsAg阳性、HBeAg阴性的血清样本中,L HBS阳性率为65.00%,HBV-DNA的阳性率为68.33%,两者阳性率差异统计学意义(χ2=47.21,P<0.001)。L HBS水平与HBV-DNA拷贝数呈直线正相关性(r=0.965)。结论L HBS和HBV-DNA的检测敏感新有差异,但相关性较强。L HBS可检测感染者体内病毒复制程度,特别是对HBeAg阴性的患者。

Objective To study the correlation of hepatitis B virus large surface protein（L HBS） and HBV-DNA and to discover the clinical significance of L HBS.Methods Enzyme linked immunosorbent assay（ELISA） and Real-time PCR were used to detect the L HBS and HBV levels,respectively.Results In 218 HBsAg and HBeAg positive samples,the positive rate of L HBS and HBV DNA were 95.41% and 92.66%,respectively.It showed significant difference between two assays（χ2=81.36,p〈0.001）.In 120 HBsAg positive and HBeAg negative samples,the positive rate of L HBS and HBV DNA were 65.00% and 68.33%,respectively.It showed significant difference between two assays（χ2=47.21,p〈0.001）.The reading of L HBS OD was positively correlated with HBV DNA copies（r = 0.965）.Conclusion As clinical indicator,the L HBS and HBV-DNA shouwed significant difference,but well correlated.L HBS level can be used to reflect the state of HBV-DNA replication in patients,especially in patients with HBeAg negative.