摘要
目的研究2009年长春婴幼儿病毒性腹泻的病原学特点。方法 2009年1-12月在长春市儿童医院共采集腹泻患儿的粪便标本419份,对其进行轮状病毒、杯状病毒、肠道腺病毒和星状病毒的检测分析。结果 419份标本中,采用ELISA法检测出轮状病毒184例,阳性率为43.91%(其中G基因型分型151例,以G3型为主,占64.24%);P基因型分型118例(其中以P8型为主,占92.37%);G/P优势组合型以G3P8为主,占48.75%(78/160)。采用RT-PCR法检测杯状病毒阳性67份,阳性率为15.99%;肠道腺病毒阳性8份,阳性率为1.91%;星状病毒阳性8份,阳性率为1.91%。结论轮状病毒是2009年长春地区婴幼儿病毒性腹泻的主要病原体,主要血清型为G3P8型;杯状病毒、星状病毒和肠道腺病毒也是重要的病原体。
Objective To study the etiology characteristics of virus diarrhea among infants by surveillance in Changchun.Methods 419 fecal specimens of diarrhea patients were collected in Changchun Children's Hospital.Rota—virus(RV),human calicivirus(HuCV),astrovirus(AstV)and adenovirus(AdeV)were determined by ELISA and PCR.Results Among 419 specimens,184 were positive 43.91% by ELISA;151 specimens of rotavirus G were tested by genotyping,G3 was the predominant type(97/151;64.24%);Among 118 specimens of genotype P,P8 was the predominant type(109/118;92.37%);G3P8was the predominant type in G/P dominant combination type(78/160;48.75%).There were 67 cases HuCV infections(15.99%) by RT-PCR,8 cases AstV infections(1.91%),and 8 cases AdeV infections(1.91%).Conclusion RV is the dominance etiological agent of infantile diarrhea in Changchun,the predominant serotype is G3P 8;HuCV,AstV and AdeV are also the important etiological agents.
出处
《中国卫生工程学》
CAS
2012年第4期337-338,340,共3页
Chinese Journal of Public Health Engineering