新疆不同来源单核细胞增生李斯特氏菌血清型的多重PCR鉴定

Xinjiang Strains of Listeria spps of Diverse Origins Serotyped by Multiplex PCR

为鉴定分离自新疆北疆绵羊单核细胞增生李斯特氏菌,本研究采用多重PCR方法,鉴定来自病发地区部分羊场的发病绵羊、健康绵羊、羊舍环境和乌鸦粪分离的30株李斯特氏菌分离株的8株单核细胞增生李斯特氏菌分离株血清型。结果为4株发病绵羊株有3株鉴定为单核细胞增生李斯特氏菌,血清型为1/2a或4b,1株为非单核细胞增生李斯特氏菌;5株健康绵羊株血清型为1/2a;其余来自羊舍水源的3株、乌鸦粪的2株及健康绵羊16株为非单核细胞增生李斯特氏菌,表明来自发病绵羊、健康绵羊及参考菌株LM血清型之间具有相关性。

To identicate Listeria monocytogenes isolates from North of Xingjiang sheep,8 Listeria monocytogenes isolates of 30 Listeria spps isolates,isolated from diseasing sheep,healthy sheep,environment and bird droppings at sheep farms in epidemic areas of Listeriosis were identified and serotyped by multipelx PCR method.The results showed that among 4 strains of Listeria isolated from diseasing sheep,3 strains were serovar 1/2a or 4b of Listeria monocytogenes,and 1 strain was not Listeria monocytogenes;5 strains from healthy sheep serovar were 1/2a;3 strains isolated from water,2 strains from crow droppings and 16 strains of Listeria monocytogenes isolated from healthy sheep were not isolates of Listeria monocytogenes.The study suggested that there was correlativity among serovars of strains of Listeria monocygenes from diseasing sheep,healthy sheep and reference strain Listeria monocytogenes.