高胆红素血症新生儿先天性巨细胞病毒感染检测

Detection of congenital cytomegalovirus infection in newborns with hyperbilirubinemia

目的了解高胆红素血症新生儿巨细胞病毒(HCMV)感染情况并分析其意义。方法对170例高胆红素血症新生儿血清标本检测抗HCMV-IgG和IgM及血清总胆红素(TBIL),以排除高胆红素血症新生儿(100例)血清为正常对照;对筛查HCMV-IgM阳性的标本进一步分析DNA含量。结果高胆红素血症患者血清HCMV-IgG、IgM阳性率分别为57.6%和13.5%,正常对照组为42.0%和3.0%,差异均有统计学意义(P<均0.05);单个核细胞DNA阳性率86.9%,血浆游离DNA阳性率13%,差异有统计学意义。结论 HCMV感染与新生儿高胆红素血症相关性大,可能是引起高胆红素血症的主要病原之一;且HCMV DNA主要分布于单个核细胞中。

Aims To ascertain the incidence of hyperbilirubinemia and neonatal cytomegalovirus（HCMV） infection and analyze their significance.Methods Anti-HCMV-IgG and IgM and serum total bilirubin（TBIL） were detected in serum samples from 170 infants with neonatal hyperbilirubinemia.Serum samples from infants with no neonatal hyperbilirubinemia（100 infants） served as a normal control.DNA was further analyzed to screen for HCMV-IgM positive samples. Results Sera from infants with hyperbilirubinemia tested positive for HCMV-IgG and IgM at a rate of 57.6% and 13.5%,respectively,while the normal controls tested positive at a rate of 42% and 3%,respectively.The differences were statistically significant（P0.05）.Positivity for mononuclear DNA was 86.9%,while positivity for free DNA in plasma was 13%.The differences were statistically significant（P0.05）.Conclusion There is a substantial association between HCMV infection and neonatal hyperbilirubinemia.HCMV may be one of the main pathogens causing hyperbilirubinemia.HCMV DNA was mainly distributed in mononuclear cells.