利用种子萌发法快速检测抗草铵膦转基因大豆

Rapid Detecting Glufosinate-Tolerant Transgenic Soybean via Seed Germination Method

转基因后代植株遗传存在不稳定性,能够快速有效地筛选后代阳性植株成为转基因研究工作的重点。通过在培养基中添加不同浓度的草铵膦进行种子萌发试验,结果普通受体大豆品种东农50在大于5.0 mg.L-1草铵膦筛选压力下,种子萌发率显著降低,植株根系生长受到抑制,基本不能完成正常的生活史;携带bar基因的Southern拷贝T2代转基因大豆种子在添加5.0 mg.L-1草铵膦的MS培养基中34.7%能正常萌发生长,利用CaMV35S和bar 2对引物对5.0 mg.L-1草铵膦筛选得到的转基因植株进行检测,能扩增出正确的条带,结果均为阳性。研究表明应用种子萌发法能够快速检测草铵膦转基因大豆。

We need to choose a repaid and effective method to solve the problem that transgenic offsprings are genetic instable.In this study,the seeds of Dongnong 50 were sowed in MS medium contained different concentrations of glufosinate.Under 5.0 mg·L-1 glufosinate,the germination rate and growth of non-transgenic recipient Dongnong 50 were seriously inhibited and couldn’t complete life cycle,while 34.7% of the transgenic soybean could not only germinate and grow up,but also could amplify the right specific segment using CaMV35S and bar primers.We can deduce that the germination method is a rapid and effective method to detect glufosinate-tolerant transgenic soybean.