CO_2倍增和UV-B辐射增强对2个大豆突变体部分抗性指标的影响

Effects of Doubled CO_2 and Enhanced UV-B Radiation on Resistant Indexes in Soybean(Glycine max Merr.)

以根瘤超着生大豆突变体0498和完全不结瘤的大豆突变体0701为材料,在人工气候室内模拟条件下,研究大气CO2浓度倍增、UV-B辐射增强以及复合逆境胁迫条件对大豆部分抗性指标的影响。结果表明,大豆突变体0701在上述3种处理下,SOD活性分别下降14.5%、17.4%和25.5%;丙二醛含量在CO2倍增处理下降低29.7%,在UV-B增强处理下则升高18.4%。大豆突变体0498在上述3种处理下,UV-B吸收物质的量分别增加44.4%、39.0%和71.9%;POD酶活分别增加27.3%、47.7%和65.6%;SOD活性在UV-B增强处理下增加27.3%;丙二醛含量在UV-B增强处理下升高85.3%,在复合处理下仅升高62.7%;UV-B增强使其离子渗透率增加12.7%。综合分析可见,突变体0498的抗性优于0701;CO2倍增能提高大豆的抗性,UV-B增强则引起大豆的抗逆反应,复合处理下大豆的响应则较单一处理复杂,无明显规律。

The increases of atmospheric CO2 concentration and ultraviolet-B radiation（UV-B）have significantly affected the crop growth,development and physiological processes.In this study,the influences on some resistant indexes were analyzed under the condition of doubled CO2 concentration,enhanced UV-B irradiation and their combination.Related experiments were carried out in the artificial climate room using super-nodulating mutant 0498 and non-nodulating soybean mutant 0701 as materials.The results showed that,for mutant 0701,the activities of SOD under the three treatments were decreased by 14.5%,17.4% and 25.5%,respectively;the content of MDA was decreased by 29.7% under doubled CO2 concentration but increased by 18.4% under enhanced UV-B irradiation.For mutant 0498,under the three treatments,UV-B absorbing compounds were increased by 44.4%,39.0% and 71.9%,respectively;POD activities were increased by 27.3%,47.7% and 65.6%,respectively;the activity of SOD under enhanced UV-B was increased by 27.3%;the content of MDA was increased by 85.3% under enhanced UV-B,while only increased by 62.7% under the treatment of combination;the electrolyte leakage was increased by 12.7% only under enhanced UV-B.In conclusion,the resistance of 0498 was higher than 0701.Doubled CO2 could enhance the resistance of soybean while enhanced UV-B caused significant response.However,the responses under the treatment of combination were more complex.