顺铂与氯霉素联用增强对人肺腺癌A549细胞的抑制作用

The interaction between cisplatin and chloramphenicol on human lung adenocarcinoma cell line A549

目的体外观察顺铂和氯霉素对人肺腺癌A549细胞的相互作用。方法采用MTT法,利用中效原理判定联合应用顺铂及氯霉素对人肺腺癌A549细胞的效应。结果顺铂与氯霉素联用可以明显抑制人肺腺癌A549细胞的生长,两药单用和联用时随着药物浓度增加,其效应也增加;两药联用时的中效浓度明显小于两药单独使用时的中效浓度(顺铂减少43.8%,氯霉素减少79.1%);两药大剂量合用时为拮抗效应(CI>1),小剂量合用时为协同效应(CI<1);并且两药合用时的效应与给药的时间先后次序无关(P>0.05)。结论顺铂与氯霉素的联用可以明显抑制人肺腺癌A549细胞的生长,两药联用时的中效浓度较单用时明显减小;上述两种药合用时大剂量为拮抗,小剂量为协同;两药合用时的效应与给药的时间先后次序无关。

Objective To observe the interaction between cisplatin and chloramphenicol on human lung adenocarcinoma cell line A549.Methods The anticancer effect of the drugs was detected with MTT.The median-effect principle was used.Results The combination of cisplatin and chloramphenicol significantly inhibited the growth of human lung adenocarcinoma cell line A549.Anticancer effects of the individual and combined drugs enhanced as drug concentration increased.The 50% inhibited concentration of each drugs in combination was lesser than the concentration of each individual drugs.（Cisplatin decreased of 43.8%,Chloramphenicol decreased of 79.1%）.The combination of the two drugs at higher concentration the CI was more than 1,and the combinations at lower concentration the CI was less than 1.The sequence of administration did not influence the anticancer effects（P〉0.05）.Conclusion The combination of cisplatin and chloramphenicol significantly inhibited the growth of human lung adenocarcinoma cell line A549.The 50% inhibited concentration of each drugs in combination was lesser than the concentration of each individual drugs.The combined drugs interaction was antagonistic at higher concentration and synergistic at lower concentration.The sequence of administration did not influence the anticancer effects.