灌服中药骨碎补、丹参对大鼠正畸牙移动过程中骨密度的影响

Effect of Rhizoma Drynariae and Salvia on alveolar bone density of rats with orthodontic tooth movement

目的:探讨中药骨碎补、丹参在大鼠正畸牙移动过程中对牙槽骨密度降低的抑制作用。方法:选取72只8周龄、SPF级健康雌性Wistar大鼠,建立正畸牙移动模型,随机分为骨碎补组、丹参组和生理盐水组,每组24只。骨碎补组和丹参组每天分别灌服中药药液6 g/kg,生理盐水组每天灌服0.9%生理盐水3 mL。镍钛螺旋弹簧每周加力1次。3组动物于正畸加力第7、14、21、28天分批处死,每组分别处死6只。分离上、下颌骨,分别测量牙移动距离和牙槽骨密度,采用SPSS18软件包对数据进行统计学分析。结果:骨碎补组、丹参组牙移动距离无显著差异(P>0.05),但两者均大于生理盐水组,差异有显著性(P<0.05);骨碎补组、丹参组牙槽骨密度无显著差异(P>0.05),但两者均大于生理盐水组,差异有显著性(P<0.05)。结论:骨碎补、丹参水煎液在加速正畸牙移动的同时,均能够减缓牙槽骨密度的降低。

PURPOSE： To detect and compare the effects of traditional Chinese drugs,Rhizoma Drynariae and Salvia,on the density of alveolar bones of rats through animal models for molar movement.METHODS： 72 female,8-week-old SPF Wistar rats were selected to establish the animal models for orthodontic tooth movement.The rats were randomly divided into 3 groups： Rhizoma Drynariae group,Salvia group and normal saline（NS） group.Each group had 24 rats.Rhizoma Drynariae asperoides decoction（6 g/kg）,Salvia decoction（6 g/kg） and 3 mL 0.9% NS were drenched daily to the rats in the Rhizoma Drynariae group,the Salvia group and the NS group,respectively.Stability of the appliances was checked every day and the applied force was raised by adjusting the springs every 7 days.The rats were executed in batch on the 7th,14th,21st,28th day by removing the skulls.Then the movement distance of the first maxillary tooth and the alveolar bone density were measured.All statistical analysis was performed using SPSS 18.0 software package.RESULTS： Teeth movements in the Rhizoma Drynariae group and the Salvia group was greater than the NS group（P0.05） was found between the first two ones.Though alveolar bone density showed a trend to decrease in all 3 groups,the Rhizoma Drynariae and Salvia groups decreased slowly（P0.05）.CONCLUSIONS： Rhizoma Drynariae and Salvia can both slow the alveolar bone density decrease and accelerate the orthodontic tooth movement.