nHA/BG涂层与Bio-Oss骨粉修复种植体骨缺损的实验研究

Experimental study of osseointegration of nHA/BG coating and Bio-Oss in implant defects

目的:评价生物玻璃/纳米羟基磷灰石(nHA/BG)涂层与Bio-Oss骨粉在种植体骨缺损中引导骨再生的效果。方法:选取6只Beagle犬,拔除两侧下颌前磨牙。3个月后预备种植窝,同时颊侧制造裂隙状骨缺损(2.25 mm×3 mm×4 mm)。按照分组植入种植体和骨粉,A组为nHA/BG+Bio-Oss,B组为nHA/BG+血凝块愈合,C组为微米级HA+Bio-Oss。术后2周、处死前2周和3 d分别进行四环素、钙黄绿素和茜素红荧光标记。术后8周和16周处死动物,行大体观察和组织学测量。采用SPSS13.0软件包对数据进行统计学分析。结果:3组骨-种植体结合率(BIC)在8周时分别为30%、18%、21%,16周时分别为61%、53%、46%;缺损区新骨面积(RA)在8周时分别为(2.1±0.6)mm3、(1.4±1.0)mm3、(0.6±0.1)mm3,16周时分别为(4.2±0.7)mm3、(2.2±1.2)mm3、(1.2±0.6)mm3。各组8周与16周的BIC和RA相比均有显著差异(P<0.05);8周时,A、C 2组的BIC和RA相比有显著差异(P<0.05),16周时,A、B 2组的RA相比有显著差异(P<0.05)。结论:在种植体周围2.25 mm骨缺损区,nHA/BG涂层能促进种植体-Bio-Oss替代骨-骨的骨结合。

PURPOSE： To evaluate bone regeneration in defects at titanium implants with nHA/BG coating in conjunction with Bio-Oss.METHODS： Four mandibular premolars were extracted in 6 Beagle dogs.After 3 months,buccal dehiscence-type defects（2.25 mm×3 mm×4 mm） were surgically created following implant site.The three treatment modalities were randomly allocated： nHA/BG implant/Bio-Oss,nHA/BG implant/blood clot,and mHA implant/Bio-Oss.After 8 and 16 weeks,the dogs were sacrificed respectively.A histomorphometric analysis was performed.The data was analyzed with SPSS 13.0 software package for Student’s t test and ANOVA.RESULTS：At 8-and 16-week,nHA/BG implant group revealed comparable mean BIC（30% to 18% versus 61% to 53%）.However,mHA implant/Bio-Oss group revealed significantly lower mean BIC（21% versus 46%） values than nHA/BG implant/Bio-Oss group.A significant difference was observed for the mean BIC and RA values at 8-week between nHA/BG implant/Bio-Oss group and mHA implant/Bio-Oss group.CONCLUSIONS： It is concluded that the application of Bio-Oss graft did not seem to interfere with the nHA/BG coating activity,but ensured a stabilization of the newly formed bone for defects.