慢性乙型病毒性肝炎患者趋化因子IP-10、Rantes与氧化损伤的关系研究

Correlationship between ehemokines and oxidative stress in chronic hepatits B

目的观察慢性乙型病毒性肝炎（CHB）患者血清趋化因子IP-10、Rantes和氧化损伤的改变，探讨趋化因子和氧化应激在CHB发病机制中的作用。方法采集70例CHB患者和10例健康志愿者血液标本，采用ELISA法检测血清趋化因子IFN-叮诱导蛋白10（IP-10）、正常T细胞表达分泌的活化调节因子（Rantes）表达水平及还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）水平，并进行相关性分析。结果CHB患者血清IP-10、Rantes水平均明显高于健康对照者，并且IP-10、Rantes水平分别与丙氨酸转氨酶水平呈正相关。CHB患者GSH、GSH／GSSG水平均明显低于健康对照者，降低的GSH、GSH／GSSG水平分别与患者ALT水平呈负相关。IP-10、Rantes水平分别与GSH、GSH／GSSG呈负相关。结论趋化因子和氧化应激相互作用，参与慢性乙型病毒性肝炎的发生和发展。

Objective The aim of this study is to investigate the possible associations of chemokines IP-10, Rantes and oxidative stress in chronic hepatits B （CHB）. Methods 70 CHB patients and 10 heahhy controls were enrolled in the study. Enzyme-linked immunosorbent assay （ELISA） was used to detect the serum levels of IFN-γ-inducible protein-10 （IP-10） and regulated on activation normal T-cell- expressed and secreted （Rantes） and oxidative stress parameters （glutathione, GSH; glutathione disulfide, GSSG）. Correlationship were analyzed by Spearman＇s rank correlation. Result The levels of IP-10 and Rantes were higher in CHB patients than healthy controls, and strong positive associations were found between IP-10/Rantes and alanine aminotransferase （ALT）. The levels of GSH and GSH/GSSG were lower in CHB patients than healthy controls, and GSH and GSH/GSSG were negatively correlated with ALT. The levels of IP-10 and Rantes were negatively correlated with GSH and GSH/GSSG respectively. Conclusion Strong associations were found between chemokines and oxidative stress which participated in the pathogenesis of CHB.