手术对喉鳞状细胞癌血清中抗survivin抗体表达的影响及其与p53和c-myc自身抗体联合检测的意义

Effects of surgery on expressions of serum anti-survivin antibody and significance of combined detection of anti-survivin,anti-p53 and anti-c-myc antibody in laryngeal squamous cell carcinoma

目的:了解喉鳞状细胞癌(Laryngeal squamous cell carcinoma,LSCC)患者手术前后血清中抗survivin抗体的变化,并分析抗survivin抗体、抗p53抗体和抗c-myc抗体联合检测在LSCC术后监测中的作用。方法:酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)用于检测血清样本中抗survivin抗体、抗p53抗体和抗c-myc抗体的表达。结果:(1)在接受根治性手术治疗后,抗survivin抗体、抗p53抗体和抗c-myc抗体的表达水平显著下降。(2)抗survivin抗体在LSCC患者外周血清中高表达,特别是有颈淋巴结转移的样本。(3)抗survivin抗体、抗p53抗体和抗c-myc抗体单独检测时均获得了较高的阳性率;3种抗体联合检测的阳性率并不优于抗survivin抗体的阳性率,但优于抗p53抗体和抗c-myc抗体。结论:抗survivin抗体可能是LSCC的一种有价值的生物标记物。抗survivin抗体、抗p53抗体和抗c-myc抗体联合检测在手术治疗效果监测方面有积极意义。

Objective： To exam the serum anti-survivin antibody levels in post-surgery and pre-surgery samples of patients with laryngeal squamous cell carcinoma（LSCC） and to investigate the potential use of anti-survivin,anti-p53,and anti-c-myc antibodies in the monitoring of LSCC postoperatively. Methods：Enzyme-linked immunosorbent assay （ELISA） was used for the detection of serum anti-survivin, anti-p53, and anti-c-myc antibodies. Results： （1）After radical surgical treatment,the anti-survivin, anti-p53, and anti-c-myc antibodies expression levels were decreased significantly. （2）High levels of anti-survivin antibody were detected in the serum sample of patients with LSCC,especially in samples from individuals with cervical lymphatic metastasis. （3）High positive detection rates were obtained through independent testing of anti-survivin, anti-p53 and anti-c-myc antibodies;the combined positive rate of all three antibodies was inferior to that of anti-survivin antibody but superior to that of anti-p53 and anti-c-myc antibodies. Conclusions：This indicates that anti-survivin antibody may be a biomarker of LSCC. Combined testing of three different tumor markers may be valuable for monitoring of the surgery efficacy.