睾酮对雄兔膝骨关节炎预防作用的实验研究

Experimental study on preventive effects of testosterone on knee osteoarthritis in male rabbits

目的:探讨睾酮对雄兔膝骨关节炎(Osteoarthritis,OA)预防的作用。方法:将18只雄性家兔随机分为去势组、未去势组和睾酮补充组,每组6只,未去势组切除右膝关节前交叉韧带和内侧半月板,去势组和睾酮补充组在切除右膝关节前交叉韧带和内侧半月板同时切除双侧睾丸,睾酮补充组于术后第3周开始肌注十一酸睾酮,6 mg/kg,每2周1次,共3次,正常喂养8周后处死所有家兔取右股骨内侧髁行大体评估、切片用免疫组织化学法分析测定软骨细胞类胰岛素1号增长因子(Insulin-likegrowth factors,IGF-1)表达和关节软骨Mankin评分,测定不同时期血清总睾酮。结果:未去势组和睾酮补充组软骨细胞中IGF-1的表达高于去势组(P<0.05)、Mankin评分明显低于去势组(P<0.05),未去势组和睾酮补充组4周和8周时血清总睾酮明显高于去势组(P<0.05),差异有统计学意义。结论:睾酮对雄兔膝OA有预防作用。

Objective：To investigate the preventive effect of testosterone on knee osteoarthritis in male rabbits. Methods：Eighteen male rabbits were randomly divided into castration group,noncastration group and testosterone supply group（n=6）. The rabbits in cas- tration group underwent the excision of anterior cruciate ligment and the medial meniscus on the right knee joint while those in non- castration group and testosterone supply group underwent the excision of bilateral testicles along with the treatment in castration group. Testosterone undecanoate（6 mg/kg,once every two weeks, three times in total） was injected intramuscularly to rabbits in testosterone supply group at the third week after the operation. All rabbits were killed by gas embolism at the eighth week. Macroscopic evaluation was performed on the articular cartilage of medial malleolus of femur. Insulin-like growth factors（IGF-1） immunohistochemical examination was performed in order to investigate the expression of IGF-1 in articular cartilage and the im- proved Mankin＇s scores was applied to test the serum levels of total testosterone at different stages. Results：Expressions of IGF-1 were higher in noncastration group arid testosterone supply group than in castration group（P〈0.05）. Mankin＇s scores were lower in noncastration group and testosterone supply group than in castration group（P〈0.05）. The serum level of testosterone was lower in cas- tration group than in noncastration group and testosterone supply group at the fourth arid eighth week （P〈0.05）. Conclusion：Testos- terone has preventive effect on knee osteoarthritis in male rabbits.