核周型抗中性粒细胞胞浆抗体在溃疡性结肠炎诊断中的意义

The significance of perinuclear anti-neutrophil cytoplasmic antibodies in diagnosis of ulcerative colitis

目的了解血清中核周型抗中性粒细胞胞浆抗体(pANCA)对溃疡性结肠炎诊断的敏感性及特异性。方法本文采用间接免疫荧光法对我院51例溃疡性结肠炎、15例克罗恩病、30例对照患者血清中核周型抗中性粒细胞胞浆抗体进行检测。结果 51例溃疡性结肠炎患者中32例核周型抗中性粒细胞胞浆抗体阳性,其敏感性及特异性分别为62.75%及95.56%。15例克罗恩病患者中有2例阳性核周型抗中性粒细胞胞浆抗体阳性,阳性率为13.33%,30例对照组未发现核周型抗中性粒细胞胞浆抗体阳性患者,组间差异具有统计学意义(P<0.05)。结论血清中抗中性粒细胞胞浆抗体对溃疡性结肠炎具有一定的敏感性及高度的特异性,对其检测有利于溃疡性结肠炎的诊断及鉴别诊断。

Objective To study the sensibility and specificity of detection of perinuclear anti-neutrophil cytoplasmic antibodies （pANCA） in diagnosis of ulcerative colitis （UC）. Methods Serum samples were obtained from 51 patients with ulcerative colitis （UC）, 15 patiens with Crohn＇ s disease （CD） and 30 patients without intestinal disease as con- trol. pANCA was detected by indirect immunofluorescence assay. Results The sensibility and specificity of p-ANCA in UC patients were 67.75% and 95.56% respectively, but the positive of pANCA in CD patients was 13.33% （2/15）. No patients was positive in control group （P 〈 0.05）. Conclusion Serum pANCA in patients with UC shows a moder- ately sensibility and a high specificity, it is useful for the diagnosis of UC and differentiation between UC and Cl）.