HCV/HIV-1合并感染者血清GP73变化特征

Variation characteristics of serum GP73 in Patients with HCV/HIV-1 co-infection

目的观察合并艾滋病的丙型肝炎患者血清GP73水平的变化特征。方法本研究主要观察了3个人群:50例健康体检者;22例AIDS患者,其中10例患者合并慢性HCV感染;28例没有合并症的单纯慢性丙型肝炎的患者。血清GP73采用标准ELISA法检测。结果与健康对照人群(36.18±12.26)ng/mL相比,慢性丙型肝炎患者血清GP73(79.73±43.91)ng/mL显著升高(P<0.0001);而AIDS患者血清GP73(122.6±65.51)ng/mL较单纯慢性丙型肝炎患者也显著升高(P=0.008)。对22例AIDS患者血清GP73分析显示,合并丙型肝炎的患者(98.32±60.14)ng/mL,低于单纯HIV-1感染的患者(142.8±65.23)ng/mL,但两组之间差异并无统计学意义(t=1.65,P=0.11)。AIDS患者的血清GP73水平与CD4+T和CD8+T细胞的绝对计数呈显著负相关(r值分别为-0.58和-0.49),而与HIV RNA拷贝数成正相关(r=0.61,P=0.0026)。结论合并HIV-1感染可以显著增加丙肝患者血清GP73水平。检测血清GP73水平的变化在一定程度上可以反映HIV-1复制情况及免疫状态的变化。

Objective To investigate the Variation characteristics of serum GP73 in HCV/HIV-1 co-infected pa- tients. Methods Three study groups included in this study： 50 healthy individuals as control; 22 HIV patients inclu- ding 10 patients with HCV/HIV-1 co-infection; 28 chronic HCV-infected patients. Serum GP73 was measured with ELISA according to the protocol. Results The serum GP73 concentration in chronic HCV-infeeted patients （79.73 _＋ 43.91 ） ng/mL was significantly higher than that in healthy individuals （36.18 _＋ 12.26） ng/mL （P 〈0.0001） ; but the level of GP73 in chronic HCV-infected patients was significantly lower than that in HIV-l-infi,eted patients （ 122.6 _＋ 65.51） ng/ml, （P=0.008）. The serum GP73 concentration in HCV/HIV-1 co-infected patients was （98.32 ＋_ 60. 14 ） ng/mL, which was lower than that in HIV-1 infected patients （ 142.8 ＋ 65.23） ng/ml,, but the difference was not significant （t = 1.65 ,P =0.11 ）. More importantly, serum GP73 protein in HIV-1 intected patients was nega- tively related with CD4＋ cell counts （r = -0.58） and CD8＋ cell counts（r = -0.49）, and positively related with HIV viral loads （r = 0.61 ,P = 0. 0026）. Conclusion Serum GP73 in HCV/HIV-1 co-infected patients was significantly in- creased, and changes of serum GP73 to a certain extent reflect the HIV-1 replication and immune status.