人增殖性糖尿病视网膜病变纤维血管膜中Visfatin的表达研究被引量：2

VISFATIN EXPRESSION IN HUMAN FIBROVASCULAR MEMBRANES OF PROLIFERATIVE DIABETIC RETINOPATHY

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摘要目的:观察Visfatin在人增殖性糖尿病视网膜病变(PDR)病人纤维血管膜中的表达,探讨其在PDR中可能的作用机制。方法:收集16例行玻璃体手术的增殖性糖尿病视网膜病变病人术中取出的纤维血管膜(Fibrovascular membranes,FVMs),应用免疫荧光方法检测Visfatin和GFAP的蛋白表达及定位,TR-PCR检测Visfatin在纤维血管膜中mRNA表达。结果:Visfatin免疫荧光结果显示在所有16例血管膜中均有强阳性表达且与GFAP共表达,RT-PCR显示在检测的8例中7例强表达。结论:Visfatin在PDR病人FVMs的表达可能与增殖性糖尿病视网膜病变纤维血管膜的形成相关,Visfatin可能成为治疗PDR的新靶点。 Objective：To investigate the expression of visfatin in human fibrovascular membranes （FVMs） of proliferative diabetic retinopathy （PDR） patients, and try to explore the possible role of visfa- tin in the occurrence and development of PDR. Methods： FVMs samples were collected from 16 PDR patients by vitrectomy. Visfatin and GFAP expression in the membranes were examined by immunofluo- rescence analyses;total mRNA was isolated from the samples and detected by RT -PCR. Results： Im- munofluorescence analysis showed that visfatin and GFAP were co - expressed in most of the ceils in the FVMs and visfatin mRNA was detectable. Conclusion： Visfatin was detectable at both protein and mRNA levels in all excised fibrovascular membranes, which suggests that it may play a role in the path- ogenesis of fibrovascular membranes in PDR patients.

作者杨晓静崔巍高伟路强

机构地区内蒙古中蒙医院眼科内蒙古自治区人民医院眼科

出处《内蒙古医学院学报》 2012年第4期285-288,共4页 Acta Academiae Medicinae Neimongol

基金内蒙古自治区自然科学基金(2009MS1111) 内蒙古自治区人民医院科研基金(20110929 20110907)

关键词 VISFATIN VEGF 糖尿病视网膜病变 visfatin fibrovascular membranes proliferative diabetic retinopathy

分类号 R587.2 [医药卫生—内分泌]

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1Li Calzi S, Neu MB, Shaw LC, et al. Endothelial progenitor dysfunction in the pathogenesis of diabetic retinopathy: treatment concept to correct diabetes - associated deficits [J]. EPMA J,2010;l(1) :88 - 100.
2Moschen AR, Kaser A, Enrich B, et al. an adipocytokine with proin - flammatory and immunomodulating properties [ J ]. J lmmunol, 2007 ; 178 ( 3 ) : 1748 - 1758.
3Dominik GH, Ammon H, Angela S, et al. Visfatin Response to Glucose Is Reduced in Women With Gestational Diabe- tes Mellitus [ J ]. Diabetes Care, 2007 ; 30 ( 7 ) : 1889 - 1891.
4Guang S,Jessica B, Sammy K,et al. Serum visfatin concen- trations are positively correlated with serum triacylglycerols and down - regulated by over feeding in healthy young men [ J ]. Am J Clin Nutr, 2007 ;85 ( 2 ) :399 - 404.
5Kim SR, Bae SK, Choi KS, et al. Visfatin promotes angio- genesis by activation of extracellular signal - regulated ki-nase 1/2 [ J ]. Biochem Biophys Res Comrnun, 2007 ; 357 (1) :150 - 156.
6Rungger BE, Dosso AA, Leuenberger PM. Glial reactivity, an early feature of diabetic retinopathy [ J ]. Invest Ophthal- mol Vis Sci,2000;41(7) :1971 - 1980.
7Amin RH, Frank RN, Kennedy A,et al. Vascular endotheli- al growth factor is present in glial cells of the retina and optic nerve of human subjects with nonproliferative diabetic retinopathy [ J ]. Invest Ophthalmol Vis Sci, 1997 ;38 ( 1 ) : 36 -47.
8Xiao J, Xiao Z J, Liu ZG,et al. Involvement of dimethylargi- nine dimethylaminohydrolase- 2 in visfatin -enhanced an- giogenic function of endothelial cells [ J ]. Diabetes Metab Res Rev,2009 ;25( 3 ) :242 -249.
9Adya R, Tan BK, Punn A, et al. Visfatin induces human endothelial VEGF and MMP - 2/9 production via MAPK and PI3K/Akt signalling pathways : novel insights into vis- fatin - induced angiogenesis [ J ]. Cardiovasc Res, 2008 ; 78 (2) :356 -365.
10Lovren F, Pan Y, Shukla PC, et al. Visfatin activates eNOS via Akt and MAP kinases and improves endothelial cell function and angiogenesis in vitro and in vivo:trans- lational implications for atherosclerosis [ J ]. Am J Physiol Endocrinol Metab ,2009 ;296( 6 ) : 1440 - 1449.