二白连地汤对溃疡性结肠炎大鼠免疫机能的影响

Experimental Study on The Effect of Erbailiandetang in Immunologic Function of Rats with Ulcerative Colitis

目的:探讨二白连地汤对溃疡性结肠炎免疫机能的影响。方法:用葡聚糖硫酸钠(dextran sulfate sodium DSS)造模,随机分组处理,分别观察各组大鼠体重和大便性状,采用疾病活动指数(DAI)对动物状态进行评分,观察其结肠形态及病理学变化并进行评分,测定大鼠血清TNF-α、IL-10的水平。结果:模型组大鼠DAI评分、结肠形态及病理学评分明显高于正常组,血清TNF-α升高、IL-10下降。SASP组及二白连地汤组DAI评分、结肠形态及病理学评分明显低于模型组,血清TNF-α下降、IL-10升高。SASP组与二白连地汤组DAI评分、结肠形态及病理学评分,血清TNF-α、IL-10均无显著性差异。结论:二白连地汤可抑制UC大鼠TNF-α水平,提高IL-10水平,有利于炎症的消除和组织修复,能有效治疗UC。

Objective：Observe the effect of erbailianditang in immunologic function on ulcerative colitis.Methods：Induce the model of ulcerative colitis rats with DSS.Randomly divided into groups and treated.The weight and stool viscosity were observed and accumulated as disease active index（DAI） score;Detect the configuration and pathological change in colon of all rats,and examine the level of IL-10,TNF-α in serum.Results：Compared normal group,the mark of DAI,configuration and pathological change in model group had a significant increase,and the level of TNF-α in serum was also increased while IL-10 was decreased.Compared with model group,the mark of DAI,configuration and pathological change in SASP and erbailianditang group had a significant increase,and the level of TNF-α in serum was also decreased while IL-10 was increased.Compared with SASP group,the mark of DAI,configuration,pathological and the level of TNF-α and IL-10 in Group E had no significant difference.Conclusion：Erbailianditang could increase the level of IL-10,decrease the level of TNF-α,correspondingly correct the abnormal immunity function,help clear inflammation and repair tissue,finally effectively treat the ulcerative colitis rats.