高效液相色谱法测定板蓝根中fructopyrano-(1→4)-glucopyranose的含量

HPLC-ELSD Determination of Fructopyrano-(1→4)-glucopyranose in Radix Isatidis

目的建立板蓝根中fructopyrano-(1→4)-glucopyranose(FG)的高效液相色谱-蒸发光散射器含量测定方法。方法色谱柱为Agilent XDB-C18(4.6 mm×150 mm,5μm),流动相为甲醇-水(5∶95),漂移管温度为115℃,载气流速为3.0 L.min-1。结果在5 min内可完成对FG的测定,FG在8.40～21.00 ng内,其对数值与峰面积对数值线性关系良好(r=0.999 0),加样回收率为97.27%～102.72%。结论此方法准确、可靠、快速、灵敏、重现性好,可用于板蓝根中FG的含量测定。

OBJECTIVE To establish an HPLC-ELSD method to determine a new compound named Fructopyrano-（1-4）-glucopyranose in Radix Isatidis. METHODS The HPLC method was conducted on an Agilent XDB-Cls column （4. 6 mm x 150 ram, 5 μm） , with methanoL-water （5： 95） as the mobile phase. The drift tube temperature was 115 ℃ and flow fate of carrier gas was 3 L. min -i. RESULTS The calibration curve was linear for FG in the range of 8.40 - 21.00 ng （ r = 0. 999 0 ）. The recovery was 97.27% - 102. 72%. CONCLUSION This method is accurate, reliable, rapid, sensitive, reproducible, and can be used for the determination of FG in Radix Isatidis.