运用代谢组学研究RSV与水稻的互作初探

A Pilot Study of Metabolites in Rice Stripe Virus Infected Rice(Oryza Sativa L.)

水稻条纹叶枯病严重威胁着我国的水稻生产,该病由水稻条纹病毒(Rice stripe virus,RSV)引起。目前,对水稻与RSV互作机制的认识还较少,这是制定有效措施来对RSV进行防控的一大障碍。代谢组学是新近发展起来的一种研究手段,它通过考察生物体系包括细胞、组织或整个生物体在受到刺激或扰动后,在代谢水平上的应答,来研究一系列的生物现象。运用代谢组学对水稻与RSV的互作进行了初步探索。GC-MS分析表明,RSV的侵染能显著影响水稻的代谢谱,且在代谢组水平上,不同抗感性的水稻对RSV的响应存在着差异。与NIST质谱数据库中的信息比较,从武育粳3号健康植株中鉴定到内源性代谢物12种、武育粳3号发病植株中11种、KT95-418健康植株中9种、KT95-418发病植株中14种。

Rice stripe disease caused by r/ce stripe virus （RSV） is a major threat to the rice production of China. One of the most important hurdles preventing the development of effective controlling measures of RSV is the lack of knowl- edge on RSV-rice interaction. Metabolomics is a newly developed research area which studies the responses of a cell, a tissue or the whole body to environmental cues at the level of metabolites. RSV infection could result in significant chan- ges in the metabolites of rice by GC-MS. At the level of metabolites, the rice cuhivars with distinct susceptibility might respond differentially to RSV. By database retrieving, 12, 11,9 and 14 kinds of metabolites were identified from Wuyu- jing 3 healthy, Wuyujing 3 diseased, KT95-418 healthy and KT95-418 diseased plants, respectively.