棉花亲环素基因GhCYP1的功能分析

Functional Analysis of Cyclophilin (GhCYP1) in Gossypium hirsutum

【目的】研究转棉花GhCYP1对烟草耐旱能力的影响。【方法】利用Gateway技术构建GhCYP1的植物表达载体pGWB-GhCYP1,采用农杆菌介导的遗传转化技术,获得融合目的基因的转基因烟草植株。以烟草野生型(WT)、L1和L2转基因系为试验材料,测定水分胁迫条件下烟草叶圆片中叶绿素的相对含量、叶片中相对水分含量、丙二醛含量和相对电导率。【结果】与野生型烟草植株相比,转GhCYP1烟草植株根系粗壮、发达。水分胁迫下转基因烟草叶圆片中的叶绿素相对含量显著高于野生型。水分胁迫3 d,WT、L1和L2中相对含水量、丙二醛含量和相对电导率无显著差异(P<0.05)。水分胁迫13 d,转基因系叶片中相对含水量明显高于野生型(P<0.05),丙二醛含量和相对电导率均明显低于野生型(P<0.05)。【结论】干旱胁迫对野生型烟草产生了较大影响,而转基因烟草显示出较强的耐旱能力,表明GhCYP1的过量表达有助于提高烟草的耐旱能力。

[ Objective ] The objective of this study is to investigate the effects of cotton GhCYP1 gene on drought tolerance of tobacco. [Method] The plant expression vector of GhCYP1 gene was constructed by using gateway cloning technology and transformed into tobacco based on Agrobacterium tumerficiens-mediated transformation approach. Transgenic tobacco plants fused the target genes were generated. Wild type （WT）, transgenic lines LI and L2 of tobacco were used to measure the relative content of chlorophyll in leaf discs, relative water content, malonyldialdehyde content and relative electrical conductivity in plants. [Result] Compared with WT, transgenic tobacco plants form a stronger root system. Under the conditions of water stress, the leaf chlorophyll was less affected in transgenic plants which had obviously higher leaf chlorophyll content in leaf discs. The 3rd day after water stress, relative water content, the malonyldialdehyde content and relative electrical conductivity in L1 and L2 transgenic lines showed no difference with that in WT. After 13 d under water stress conditions, relative water content in transgenic lines was significantly higher than in WT, but the malonyldialdehyde content and relative electrical conductivity were significantly lower than in WT. [Conclusion] These results suggested that WT tobacco plants were greatly affected under water stress conditions, and the transgenic plants were less affected. Overexpression of GhCYP1 improved the drought tolerance oftransgenic tobacco plants.