基因工程改造噬菌体应用于细菌感染的研究进展

Progress in Application of Genetically Engineered Bacteriophage to Treatment of Bacterial Infection

噬菌体作为细菌病毒,在细菌性感染尤其是多重耐药菌感染的治疗方面具有抗生素无法比拟的优势。目前人工改造噬菌体的理论和技术已趋成熟,研究者通过基因工程技术解决了噬菌体特异性高、半衰期短、释放内毒素等问题,使基因工程改造噬菌体具有了较强的临床应用潜力。本文主要就基因工程改造噬菌体在扩大宿主范围、增强抗生素疗效、延缓免疫清除、避免内毒素释放等方面所具有的优势,及其在剂量确定、细菌耐受、宿主安全性等方面可能会出现的问题进行了阐述。

Bacteriophage, a kind of bacterial virus, is of incomparable advantages in treatment of bacterial infection, especially in treatment of multi-resistance bacterial infection, as compared with antibiotics. At present, both theory and technologies in the field of artificial modified bacteriophage are mature. And the disadvantages of bacteriophage, e. g. high specificity, short half-life, endotoxin release, etc. can be solved by using the genetic engineering technology. Thus, it is of bright potentials to apply genetically engineered bacteriophage clinically. In this paper, it is analyzed about the advantages of the bacteriophage in extending host range, strengthening the effects of antibiotics treatment, postponing immuiae clearance, preventing release of internal toxin, as well as possible problems in dose determination, bacterium tolerance, host range safety.