纳豆激酶对正常大鼠凝血系统的影响

Effect of nattokinase on coagulation system in normal rats

目的通过检测正常大鼠的凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)及凝血酶时间(TT),观察纳豆激酶(NK)冻干粉(NK-1)肌肉注射和固体发酶纳豆粉(NK-2)给药方式的NK抗凝血作用。方法将80只SD大鼠随机分为空白对照组、肝素对照1组、肝素对照2组、阿司匹林对照组、NK-1注射低剂量组、NK-1注射中剂量组、、NK-1注射高剂量组[注射剂量依次为1.6万～25.9万U/(kg·d)],NK-2口服低剂量组、NK-2口服中剂量组、NK-2口服高剂量组[口服剂量依次为0.5万～7.5万U/(kg·d)],每组8只。给药后观察各组大鼠血浆APTT、TT和PT指标变化。结果与空白对照组比较,NK-1注射高剂量组、NK-2口服高剂量组和阿司匹林对照组PT明显延长(P<0.05,P<0.01);肝素对照1、2组、NK-1注射低、中、高剂量组、NK-2口服高、中剂量组和阿司匹林对照组APTT明显延长(P<0.05,P<0.01);肝素对照2组、NK-1注射高剂量组TT明显延长(P<0.01)。结论 NK-1在1.6万～25.9万U/(kg·d)剂量范围内注射给药,与NK-2在0.5万～7.5万U/(kg·d)剂量范围内口服给药,对正常大鼠均具有一定的抗凝血作用,且抗凝血作用随剂量的升高而增强。

Objective To study the anticoagulant effect of intrmuscular injection with dry nattoki- nase（NK） powder（NK-1） and solid NK powder（NK-2） by measuring prothrombin time（PT） ,ac- tivated partial thromboplastin time（APTT） and thrombin time（TT） in normal rats. Methods Eighty SD rats were randomly divided into control group,heparin control group 1 ,heparin control group 2, aspirin control group, and low, medium amd high NK-1 dose injection groups（1.6--25.9× 10^4 U/kg·d）,and oral low,medium amd high NK-2 dose groups（0.5--7.5 × 10^4 U/kg·d）,8 in each group. APTT,TT and PT were recorded in different groups after the drugs were used. Re- sults The PT and APTT were significantly longer in low,medium amd high NK-1 dose injection groups,high NK-2 dose oral group, heparin control group 1, heparin control group 2, and aspirin control group than in control group（P〈0.05, P〈0.01）. The TT was significantly longer in hep- arin control group 1 and high NK-1 dose injection group than in control group（P〈0.01）. Conclu- sion NK-1 injection（1.6--25.9× 10^4 U/kg·d） and oral NK-2（0.5--7.5× 10^4 U/kg·d） exert their anticoagulant effects in a dose-dependent manner in normal rats.