知柏地黄汤合桃红四物汤对CsA肾毒性的防护作用

Prevention of Cyclosporin A Nephrotoxicity in Rats with Zhibai Dihuang Decoction Combined with Taohong Siwu Decoction

目的:观察知柏地黄汤合桃红四物汤对CsA肾毒性模型大鼠的肾保护作用。方法:予30mg/kg剂量的CsA灌胃制作大鼠CsA肾毒性模型,同时分别予10mg/kg贝那普利、2g/mL中药药液大鼠灌胃以预防肾毒性。予4周末分别观察各组大鼠肾功能、肾组织病理变化,免疫组化观察各组大鼠肾组织TGFβ1的表达变化。结果:与模型组相比,中药组大鼠血肌酐降低(28.3±3.8 VS 33.1±5.2,P=0.052),病理显示中药组大鼠肾组织空泡变性较模型组改善[变性范围(8.2±2.2)%VS(15.6±3.7)%,P<0.05],炎症细胞浸润及间质纤维化亦减轻;免疫组化显示,中药组大鼠肾组织TGFβ1的表达降低。结论:知柏地黄汤合桃红四物汤具有保护和改善CsA慢性肾毒性大鼠肾功能的作用。其机制有可能与降低肾组织TGFβ1的表达有关。

Objective：To investigate the therapeutic effect of Zhibai Dihuang Decoction combined with Taohong Siwu Decoction on nephrotoxicity of cyclosporin A（CsA）.Methods：Rats were on low-salt diet and CsA was administered by gastric gavage at a dose of 30mg/kg once a day for 28 days.Zhibai Dihuang Decoction combined with Taohong Siwu Decoction or benazepril was given to these rats in an appropriate dose.The blood and urine sample were collected at 4 weeks and renal function and total urine protein were examined.Renal morphology was observed and immunohistochemical examination was performed to detect the expression of TGFβ1 in renal tissues.Results：Compared with model group,serum creatinine level was attenuated with the use of tranditional Chinese medcine（28.3±3.8 VS 33.1±5.2,P=0.052）.On histopathology tranditional Chinese medcine attenuated tubular cells vacuolations[（8.2±2.2）% VS（15.6±3.7）%,P0.05]as well as interstitial damage including interstitial inflammation and interstitial fibrosis.The expression of TGFβ1 was also reduced in renal tissues with the use of tranditional Chinese medcine.Conclusion：Zhibai Dihuang Decoction combined with Taohong Siwu Decoction can attenuate the structural and functional changes of kidney in CsA nephrotoxicity rats.The mechanism may be related to reducing renal TGFβ1 expression.