食管癌手术前后肺功能与血清细胞因子相关性研究

Correlation between lung function and serum cytokines before and after esophageal cancer operation

目的探讨食管癌手术前后肺功能与血清细胞因子的相关性。方法在行手术治疗的食管癌患者中随机选取47例,于手术前48 h日间清醒状态下检测用力肺活量(FVC)、第1秒用力呼气容积(FEV1)、肺最大通气量(MVV%)、肺弥散功能(DLCO)、气道阻力(RAW),同时在肘正中静脉抽取静脉血2 mL两份,用酶联免疫吸附试验(ELISA)检测白介素-6(IL-6)、白介素-8(IL-8)水平。手术后6 d再次检测患者肺功能中FEV、FVC、MVV%、DLCO、RAW指标;同时用ELISA方法测血清IL-6,IL-8水平。结果手术前后肺功能中FEV、FVC、MVV%、DLCO、RAW,IL-6、IL-8的水平差异均有统计学意义(P<0.05)。血清中IL-6、IL-8水平增高与FEV、FVC、MVV%、DLCO、RAW各指标变化存在着相关性。结论手术后患者血清细胞因子浓度的升高与肺功能下降有密切联系。患者血清IL-6、IL-8水平可作为临床评估术后肺功能的参考指标。

Objective To investigate the correlation between the lung function with serum cytokines before and after esophageal cancer operation.Methods 47 patients with esophageal carcinoma undergoing operation were randomly selected and detected the forced vital capacity（FVC）,forced expiratory volume in 1 second（FEV1）,maximal ventilatory volume（MVV%）,carbon monoxide diffusing capacity（DLCO） and airway resistance（RAW） in the daytime awakened state at preoperative 48 h.At the same time,2 specimens of venous blood 2mL were collected from the median cubital vein for detecting IL-6,IL-8 levels by ELISA.The parameters of FEV,FVC,MVV%,DLCO and RAW in PFT were redetected on postoperative 6 d.Serum IL-6 and IL-8 levels also were redetected by ELISA.Results FEV,FVC,MVV,DLCO,RAW in the lung function and serum IL-6 and IL-8 levels had statistical differences before and after operation（P0.05）.The increase of serum IL-6 and IL-8 levels had correlation with the changes of various indexes FEV,FVC,DLCO and RAW.Conclusion The elevation of serum cytokine levels is closely related with the decrease of the pulmonary function.Serum IL-6 and IL-8 levels may be used as the reference indexes for evaluating the postoperative pulmonary function in clinic.