空肠弯曲菌产肠毒素性的研究

STUDY ON ENTEROTOXIGENICITY OF CAMPYLOBACTER JEJUNI

通过间接ELISA法和CHO细胞法对 2 86株空肠弯曲菌产生细胞紧张性肠毒素 (Cyto tonicEnterotoxin ,CE)的检测 ,CE的检出率分别为 2 4 5%和 12 2 % ,间接ELISA法的敏感性明显高于CHO细胞法 (P <0 0 0 1) ,并且特异性较高 ,即证实间接ELISA法是检测CE的特异、敏感、简便的方法。以间接ELISA法检测 ,来自健康蛋鸡、健康后备蛋鸡、腹泻死亡后备蛋鸡、腹泻后备蛋鸡、腹泻死亡仔猪、腹泻仔猪、健康羔羊和腹泻患者菌株的CE检出率分别为 18 8% (12 /6 4 )、12 .5% (1/8)、4 4 .8% (30 /6 7)、2 1 2 % (11/52 )、12 .5% (1/8)、18.8% (15/80 )、0 .0 % (0 /6 )和 0 .0 % (0 /1)。本研究结果分析表明 ,不同来源的菌株在CE产生上有差异 ,CE产生与该菌生物型之间没有相关性 (P >0 5) ,CE产生与鸡的腹泻发生相关 (P <0 0 5) ,并且空肠弯曲菌检出率与CE检出率间呈正相关。

The production of cytotonic enterotoxin(CE)in 286 strains of Campylobacter jejuni was determined by indirect enzyme linked immunosorbent assay (ELISA) and CHO cell test.The rates of CE detection were 24.5% and 12.2% respectively.The ELISA method was significantly more sensitive than CHO cell test in the detection of CE (P<0.001),and its specificity was higher as well.It is confirmed that the ELISA is a specific,sensitive and convenient method for CE determination.As measured by the ELISA,the rates of CE detection were 18.8%(12/64),12.5%(1/8),44.8%(30/67),21.2%(11/52),12.5%(1/8),18.8%(15/80),0.0%(0/6) and 0.0%(0/1) in strains from healthy laying hens,healthy replacement laying hens,dead replacement laying hens with diarrhoea,replacement laying hens with diarrhoea,dead piglets with diarrhoea,piglets with diarrhoea,healthy lambs and patients with diarrhoea respectively.The analysis on the results of this study shows that there are some differences in CE production between strains from different source,that a correlation does not exist between CE production and biotype in C.Jejuni (P>0.5),and that a correlation exists between CE production and laying hens′ diarrhoea (P<0.05),also that there is a positive correlation between the rate of C.jejuni detection and the rate of CE detection.