HPLC法测定戟叶马鞭草苷在不同血浆中的蛋白结合率

Determination of the Protein Binding Rates of Hastatoside with Different Plasma by HPLC

目的:建立测定戟叶马鞭草苷在大鼠血浆、人血浆和牛血清白蛋白(BSA)中蛋白结合率的方法,并计算不同介质中的相关参数。方法:采用高效液相色谱(HPLC)法测定不同介质中戟叶马鞭草苷的浓度,并结合平衡透析法测定其蛋白结合率。结果:低、中、高浓度下,戟叶马鞭草苷的蛋白结合率分别为大鼠血浆:(19.52±4.7)%、(25.60±5.4)%、(20.91±3.9)%;人血浆:(23.12±5.7)%、(21.76±6.5)%、(24.30±7.6)%;牛血清白蛋白:(25.83±7.0)%、(27.70±9.1)%、(26.19±5.6)%。结论:本方法快速、简便、可靠,戟叶马鞭草苷与大鼠血浆、人血浆和牛血清白蛋白的蛋白结合率低,且与血药浓度无显著相关性。

OBJECTIVE： To establish a method for determining the protein binding rates of hastatoside in rat plasma, human plasma, bovine serum albumin （BSA）, and to calculate the correlate parameters of hastatoside in different mediums. METHODS： The concentrations of hastatoside in different mediums were determined by HPLC. The protein binding rates of hastatoside in differ- ent mediums were determined by equilibrium dialysis method. RESULTS： The protein binding rates of hastatoside with rat plasma, human plasma and BSA at low, middle and high concentrations were （19.52±4.7）% , （25.60 ± 5.4）% and （20.91 ± 3.9）% ; （23.12±5.7）%,（21.76±6.5）% and （24.30±7.6）%;（25.83±7.0）%,（27.70±9.1）% and （26.19±5.6）%. CONCLUSION： The method is rapid, simple and reliable, and the protein binding rates of hastatoside with rat plasma, human plasma and BSA are in low level and it is not proportionally dependent on plasma concentration of hastatoside.