ICP-MS技术测定清热解毒胶囊中常见金属元素的含量

Content Determination of Common Metal Elements in Qingre Jiedu Capsule by ICP-MS

目的:建立测定清热解毒胶囊中Fe、Cr、Mn、Ni、Cu、Zn、As、Ag、Cd、Sn、Sb、Ba、Pb13种常见金属元素含量的方法。方法:以湿法消解样品后,采用电感耦合等离子体质谱(ICP-MS)技术测定样品中金属元素的含量。结果:13种金属元素线性相关系数均>0.9990;方法准确度、精密度、稳定性均达到要求;有证参考物质榉木叶与茶叶验证方法准确度、精密度高。3批清热解毒胶囊中Cu、As、Cd、Pb的含量分别为0.7700、1.0200、0.0370、0.1400μg·g-1,0.8300、1.0500、0.0340、0.4100μg·g-1,0.8700、0.9300、0.0280、0.1200μg·g-1;Zn、Sn的含量分别为5.3000、0.0880μg·g-1,4.7100、0.1680μg·g-1,4.2400、0.2010μg·g-1。结论:本方法稳定、可靠。3批清热解毒胶囊中Cu、As、Cd、Pb的含量均符合国家中药材质量标准,Zn、Sn的含量符合国家相关食品标准,其余元素未见中药材标准限值。

OBJECTIVE： To establish the method for the content determination of 13 common metal elements, Fe, Cr, Mn, Ni, Cu, Zn, As, Ag, Cd, Sn, Sb, Ba, Pb, in Qingre jiedu capsule. METHODS： The samples were wet digested by concentrated nitric acid （HNO3）, and the contents of metal elements in wet digested samples were determined by ICP-MS. RESULTS： The lin- ear correlative coefficients for the common 13 elements were higher than 0.999 0. The accuracy, precision and stability of the meth- od were all in line with the standard; the validation method of certified reference substance the leaves of beech and tea was sound in accuracy and precision. The contents of Cu, As, Cd and Pb in 3 lots of Qingre jiedu capsules were 0.770 0, 1.020 0, 0.037 0, 0.140 0 μg.g^-1;0.830 0, 1.050 0,0.034 0,0.410 0 μg.g^-1;0.870 0,0.930 0,0.028 0,0.120 0 μg.g^-1, respectively. The contents of Zn and Sn were 5.300 0, 0.088 0μg.g^-1; 4.710 0,0.168 0 μg.g^-1;4.240 0,0.201 0 μg.g^-1. CONCLUSION： The method is steady and reliable. The contents of Cu, As, Cd and Pb in 3 lots of capsules meet national quality standards of TCM. The contents of Zn and Sn are up to national food quality standards. TCM standard limit of other elements haven＇ t been found.