土壤中解磷细菌的筛选

Screening phosphorus-solubilizing bacteria in the soil

目的:从赣州各种土壤中筛选获得解磷细菌,为微生物肥料的研究发展提供一定依据。方法:将土壤样品进行梯度稀释培养在选择性培养基中,根据溶磷圈的大小挑取单菌落作为初筛的结果,进一步通过液体培养,采用钼锑抗比色法测发酵液中可溶性磷含量进行复筛。结果:从土壤中筛选出两株菌株G-12和G-6,溶磷量分别达到4.9 mg/kg和4.6 mg/kg,与对照相比分别增加了53.1%和43.7%,初步鉴定是属于假单胞菌属的某个种。结论:今后将进一步对菌株G-12和G-6进行发酵条件的优化,并确定盆栽试验优化组合,为微生物肥料的研究提供一定依据。

Objective ：To obtain the phosphorus-solubilizing bacteria from different sorts of soil samples in Ganzhou in or- der to provide some basis to the development of microbial fertilizer. Methods：Serial dilutions of soil cultured in selective medium. Phosphorus-solubilizing bacteria were screened according to the size of phosphorus-solubilizing circle. The bacte- ria rescreened in shaked culture. Molybdenum-antimony ultraviolet colorimetry test measured the quantity of phosphorus in fermentation broth. Results ：2 strains G-12 and G-6 with ability of dissolving phosphorus 4.9 mg/kg and 4.6 mg/kg were obtained, which were 53.1% and 43.7% higher than control. Pure bacterial culture and identification showed that the two strains belonged to Pseudomona. Conclusions：The medium and study with pot experiment would pave a way for the study of microbial fertilizer.