摘要
目的 获得鸡卵黄抗体（Ｉｇｙ），并研究该抗体在鸡的黄液中表达的进程。方法 用吗啡—牛血清蛋白 结合物为模型抗原对３０周龄产蛋鸡进行免疫诱导，用ＥＬＩＳＡ间接法测定不同时间的抗体滴度。结果全程免疫过 程中白蛋白抗体最高滴度为１：１２８００，吗啡抗体最高滴度为 １：１６００，经 ５次免疫后（４２周龄）抗体滴度不再升高。结论 为使目的抗体在鸡卵黄液中持续而高质量诱导奠定基础。
Objective To obtain IgY and study on the expression of it in egg yolk,Methods The laying hens aged 3Ow were immunized with morphine-bovine serum albumin conjugate,and the IgY induced was detected by indirect ELISA.Results The highest biers of antibodies to albumin and morphine were 1: 12800 and 1:1600 respectively. However, no increase of the biers were observed in the hens(aged 42w)after being immunized with 5 doses of the conjugate.Conclusion The study laid a foundation of the induction and persistence of goal antibody in egg yolk.
出处
《中国生物制品学杂志》
CAS
CSCD
2000年第2期79-81,共3页
Chinese Journal of Biologicals
