我国首次分离的辛德毕斯病毒(XJ-160病毒株)全基因组核苷酸序列测定

The Complete Genomic Sequence of XJ-160 Virus Fisrtly Isolated in China

对我国首次分离的辛德毕斯病毒XJ 16 0病毒株全基因组核苷酸序列进行测定 ,阐明该病毒基因组与国外株的差异 ,了解其分子致病机理。测序结果显示 ,XJ 16 0病毒全基因组除 5′末端帽子结构和 3′末端多聚腺苷酸尾外共 116 2 6个核苷酸 ,编码 3731个氨基酸。非结构基因的编码区长 746 4核苷酸 (6 0nt～ 75 2 3nt) ,编码 2 486氨基酸 ,翻译成四种非结构蛋白 (nonstructuralprotein ,nsp1 4)。结构基因长 3738核苷酸 (75 6 8～ 1130 6 ) ,编码 12 45个氨基酸 ,翻译成三种结构蛋白和两个小分子肽 (capsid ,E1- 3、6k)。病毒基因组 5′末端和 3′末端及结构基因和非结构基因之间分别有 5 9个核苷酸、45个核苷酸和 32 3个核苷酸的非编码区。核苷酸序列分析表明 ,XJ 16 0病毒具备典型的甲病毒属辛德毕斯病毒基因组特征 ,与标准辛德毕斯病毒AR339病毒株相比 ,核苷酸序列存在 18%差异 ,氨基酸差异为 8%。这是我国完成的第一株辛德毕斯病毒全基因组序列测定 ,序列已输入国际基因库 (GenBankAF10 372 8)。

Our study showed that the complete genomic sequence of XJ 160 virus contains 11?626nt, exclusive of 5′cap and 3′ poly A, coding for 3?731 amino acids. The non structural region is comprised of 7?461 nt (60 7?523), coding for four non-structural proteins (nsp1 4; 2?486 amino acids ). The structural region contains 3?738 nt (7?568-1,1306), coding for 1?245 amino acids (capsid, E1-3, 6K). There are three of 59nt, 45nt, 323nt noncoding regions in 5′ end, a junction between non structural and structural regions and a 3′end. XJ 160 virus conserves all consensus sequence elements commonly found in alphaviruses. Compared with Sindbis viurs AR339 (strain HRSP), significant divergence was found in the XJ 160 sequence. Only 82% nucleotides and 91.4% amino acids were shared by the two strains. We have submitted this sequence to GenBank (AF1037280).