甘草苷对人膀胱癌T24细胞p21、PTEN的表达影响

Effects of Liquiritin on p21 and PTEN Expression in Human Urinary Bladder Cancer T24 Cells

目的:探讨甘草苷(Liquiritin,LQ)对膀胱癌细胞T24 p21、PTEN的表达影响及其可能的抗癌机制。方法:实验分组:实验分为对照组,LQ 20μg/mL,50μg/mL,100μg/mL组。用RT-PCR和RT-qPCR检测p21、PTEN的mRNA表达,Westernblot检测LQ对T24细胞内p21、PTEN蛋白的表达影响。结果:与对照组比较,LQ 20μg/mL,50μg/mL,100μg/mL组PTENmRNA和蛋白表达均升高,LQ50μg/mL,100μg/mL组p21mRNA表达升高,LQ 20μg/mL,50μg/mL,100μg/mL组p21蛋白表达明显升高,差异有统计学意义。结论:LQ能诱导T24细胞p21、PTEN的表达,其抗癌机制可能与此相关。

Objective： The present study focus on the effects of Liquiritin（LQ） on p21,PTEN expression of human bladder cancer cell line T24 and its potential antitumor mechanism.Methods： Four groups including control,LQ20μg/mL,LQ50μg/mL,and LQ100μg/mL group were set up in this study.p21 and PTEN mRNA expression of T24 cells was detected by using RT-PCR and RT-qPCR.Expression of p21 and PTEN protein was analyzed by western blot.Results： Compared with the control,after treatment of LQ of 20μg/mL,50μg/mL,100μg/mL,expression of p21 mRNA and protein both increased,and PTEN mRNA and protein also increased.Conclusions： The above results indicated that： LQ induced p21 and PTEN expression,and this maybe related antitumor effect of LQ.