利用AABBDDDD八倍体培育小麦-簇毛麦二体附加系的研究

Studies of Development of Disomic Addition Lines of Triticum aestivum-Haynaldia villosa via AABBDDDD Octaploid

对普通小麦（Triticumaestivum）-节节麦（Aegilopssquarrosa）八倍体（2n=8x=56，AABBDDDD）与硬粒小麦（Triticumdurum）-簇毛麦（Haynaldiavillosa）六倍体（2n=6x＝42,AABBVV）杂交后，将所得七倍体杂种（AABBDDV）进行连续自交，在F4代中利用C-分带鉴定出可能的簇毛麦6V二体附加系95－7和2V二体附加系26－7，其花粉母细胞染色体在减数分裂中期I的配对构型分别为0.14I＋20．42＋1．5和0．10I＋20．07＋1.82；进一步将95－7和26－7的基因组DNA用EcoRI酶切，分别用小麦族第6部分同源群短臂探针Psr113和第2部分同源群长臂探针BCD240进行Southern杂交，结果显示具有簇毛麦的特异杂交带，进一步确证了95－7和26－7分别是普通小麦-簇毛麦6V和2V二体附加系。

Hexaploid hybrid between Triticum aestivum-Aegilops squarrosa (2n = 8x =56, AABBDDDD) octaploid and Triticum durum-Haynaldia villosa (2n = 6x = 42,AABBVV) was self pollinated. The puhtative T aestivum-H villosa 6V and 2Vaddition lines were creened by C-banding in F4 and chromosome configurations ofPMC at MI were 0.141 + 20.42 + 1.50 and 0.10 I + 20.07 + 1.82 , respectively.Genomic DNA of 95-7 and 26-7 were digested by EcoRI, and Southern bloting wasemployed using group 6 probe Psr113 for 95-7 and group 2 probe BCD240 fro 26-7.The results showed that 95-7 and 26-7 had the. same special bands as H villosa did.Therefore, 95-7 and 26-7,further identified were T aestivum-H villosa 6V and 2Vdisondc addition lines.