圆背角无齿蚌血细胞在体内和体外损伤修复过程中的功能

FUNCTIONS OF HEMOCYTES OF ANODONTA WOODIANA PACIFICA(HEUDE)DURING THE PROCESS OF WOUND HEALING BOTH IN VITRO AND IN VIVO

以圆背角无齿蚌为材料，采用外套膜小片的异体移植与加入血细胞的体外培养，研究了血细胞在体内、外损伤的修复及珍珠囊构建中的功能。结果表明人工育珠手术后的损伤修复中，血细胞有形成无颗粒细胞层修复伤口、诱导表皮细胞迁移和再生并形成珍珠囊的功能。而在体外的组织培养中加入血细胞比不加入血细胞的对照组，能更快地诱导上皮细胞迁移和再生，在整个伤面形成覆盖层，即形成类似珍珠囊结构。本文首次在国内、外报道了用组织培养技术培养珍珠囊成功。

This paper reports the functions of hemocytes of Anodonta woodiana pacifica (Heude) during the process of wound healing and pearl sas formation by using pieces of mantle allograft and the mixed culture of pieces of mantle and hemocytes in vitro. After the operation of artificial pearls, the hemocytes exhibited abilihes to form layers of agranulocytes to heal wounds and induce the migration and fegeneration of epithelial cells to form pearl sac. Comparing the tissue culture experiment in which hemocytes were added with the control group without adding hemocytes it was found that the hemocytes in the former can induce more signifilcantly and rapidly the migration and regeneration of the mantle epithelial cells to form the cover layer on the whole face of wounds, which is similar to the structure of pearl sac. It is thus demonstrated that the functions of hemocytes in vitro are basically the same as those in vivo during the process of wound healing and pearl sac formation. This paper is the first to report the successful production of pearl sacs by using tissue culture technique in this field.