摘要
利用实验室自制及购买的几种脂肪酶制剂催化的酯化反应来制备亚油酸油醇酯，其中本实验室制备的丝孢酵母脂肪酸酯化效果最好，作为进一步研究的实验用酶。以正己烷为反应溶剂，在微水系统中对影响亚油酸油醉酯合成的各种因素进行了研究，确定酯化反应合成的最适温度为３５℃，０～１００℃反应１０ｈ的酯化率均可达到９０％，最适酯化ｐＨ为８．０，最适底物浓度为０．２５ｍｏｌ／Ｌ，最适水含量为０．０５％，在选用的１１种有机溶剂中，以环己烷的酯化率最高，二甲亚砜最低。
The effects of several lipases of different sources on oleyl linoleate synthesis were compared. The strain producing lipase of highest activity was screened by our laboratory. Several factors which influenced oleyl linoleate synthesis was studied. The optimum temperature for esterification was 35℃ and the esterification reaction could proceed at temperature range of 4 - 80℃, the optimum pH was 8.0, optimum substrate concentration was 0.25mol / L Among 11 kinds of organic solvents compared, the esterification rate in cyclohexane was ten times higher than that in dimethylsulfoxide.
出处
《微生物学通报》
CAS
CSCD
北大核心
2000年第3期195-198,共4页
Microbiology China
基金
华东理工大学生物反应器工程国家重点实验室开放课题
关键词
酯蜡
脂肪酶
酯化反应
微水系统
亚油酸油醇酯
Oleyl linoleate, Lipase, Esterification reaction, Microaqueous system
