应用PCR-SSCP快速鉴定结核分枝杆菌复合群

FAST DIFFERENTIATION OF MYCOBACTERIAL SPECIES BY USING PCR-SSCP

通过聚合酶链反应（ＰＣＲ）－单链构象多态性（ＳＳＣＰ）技术分析结核分枝杆菌和非结核分枝杆菌临床株的１６ＳｒＤＮＡ基因。６０例临床标本中，２０例为阳性，与传统方法比较无差异。其中分型：１８例为结核分枝杆菌，２例为结核分枝杆菌和非结核分枝杆菌双重感染。２０例阴性标本中，ＰＣＲ－ＳＳＣＰ又检查出５例阳性。２０例对照标本中，３种传统方法与ＰＣＲ－ＳＳＣＰ法均检测为阴性。全部试验３ｄ报告结果。结核分枝杆菌复合群和卡介苗的图形相同以外，其它分枝杆菌的ＰＣＲ－ＳＳＣＰ电泳图谱均有差异。所以，应用ＰＣＲ－ＳＳＣＰ技术快速鉴定结核分枝杆菌是可行的。

Methods Using PCR-SSCP analysis the 16S rDNA of mycobacterial species. In sixty clinical specimen, twenty were positive by using both traditional methods and PCR-SSCP. In these twenty species, eighteen were M. Tuberculosis and two were infected by both mycobacterial species and other than mycobacterium tuberculosis. eve of twenty species which were negative by using traditional methods were positive by using PCR-SSCP. All methods showed negative in twenty control species. All experiments reported results in three days. Using PCR-SSCP, there were different profiles in different mycobacterial species, except that there were similar profiles in mycobacterial and M. bovis BCG. It can be used to differentiate mycobacterial species by using PCR-SSCP.