Physical Mapping of the Sequences Homologous to Disease Resistance Genes myb1 and NDR1 in Maize

玉米中抗病基因myb1和NDR1同源序列的荧光原位杂交物理定位(英文)

Using fluorescence in situ hybridization, the authors investigated the homology between three plant species, maize (Zea mays L.) and tobacco (Nicotiana tabacum L.), maize and Arabidopsis thaliana (L.) Heynh. at cytogenetic level using two probes corresponding to functional disease resistance genes myb1 and NDR1 in Arabidopsis and tobacco respectively. The hybridization signals of the tested probes were detected in maize chromosomes 8 and 5 respectively, and the single location of each of the two probes showed only single copy of them in maize genome. The results provided a valuable insight into searching for genes associated with programmed cell death in plants using heterologous probe with comparative genetic approach. In addition, the improvements of FISH technique using heterologous probes were discussed.

以烟草和拟南芥中的单拷贝抗病基因myb1和NDR1作探针 ,利用荧光原位杂交的方法分别对这两个基因在玉米 (ZeamaysL .)和烟草 (NicotianatabacumL .)、玉米和拟南芥 (Arabidopsisthaliana (L .)Heynh .)中的同源性做了研究。杂交结果表明myb1和NDR1的同源序列分别位于玉米第 8、5染色体 ,单个信号位置表明这两个基因的同源序列在玉米基因组中只有一个拷贝。为通过比较遗传学方法 ,以异源程序性死亡相关基因为探针寻找不同物种中的同源基因提供了重要线索。同时对利用异源探针进行原位杂交的技术改进进行了讨论。