碱性β-甘露聚糖酶发酵培养基的优化

Optimization of Fermentation Medium for Alkaline Mannanase Production

选用魔芋粉作为碳源,豆饼粉和谷氨酸钠作为氮源物质,对发酵培养基进行优化,并进行模型验证和5L发酵罐生产实验。结果表明:最佳发酵培养基(g/L,5L发酵罐)为魔芋粉12、豆饼粉15、酵母粉5、NaCl 84.4、谷氨酸钠3.11、K2HPO41.5、MgSO40.3、Na2CO310,起始pH 9.5～10.0,碱性β-甘露聚糖酶活力最高可达到2610.1U/mL。

The fermentation medium for producing alkaline mannose using konjac flour as the carbon source and soybean cake powder and sodium glutamate as the nitrogen source was optimized by Pluckett-Burman（PB） design,steepest ascent test and central composite design.Based on a central composite design for three independent variables including NaCl and sodium glutamate concentrations,a predictive mathematical model was built and validated.Meanwhile,scale-up production experiments were done in a 5-L fermentor.Our results indicated that the optimal fermentation medium（g/L） was composed of konjac flour 12,soybean cake powder 15,yeast powder 5,NaCl 84.4,sodium glutamate 3.11,K2HPO41.5,MgSO40.3,Na2CO310,and initial pH 9.5 — 10.0,yielding an alkaline mannose as high as 2610.1 U/mL.