小麦与高冰草(长穗偃麦草)体细胞杂种株系与其亲本幼苗抗盐性的比较

Studies on NaCl-tolerant among somatic hybrid lines of wheat and Agropyron elangatum with their parents

利用不同浓度 Na Cl处理高冰草、小麦 ,小麦和高冰草体细胞杂种 F3代 - 1、 - 2的幼苗 ,5d后观察其形态 ,计算耐盐系数 ,测定生长量及脯氨酸含量 ,Na+、K+、Ca2 +等生理指标。结果表明 ,高冰草具有典型耐盐单子叶植物的特征。亲本小麦属非盐生植物 ,抗盐能力较弱 ,当 Na Cl浓度达 0 .5%时已有盐害影响 ,超过后迅速加重甚至死亡 ,而杂种受害的盐度比小麦高。 1 .0 %以上 Na Cl处理组中 ,死亡率远较小麦低 ,生长量及各生理指标的测定也表明杂种较其亲本小麦 (济南 1 77)具有较高的耐盐性。证明通过体细胞杂交高冰草与抗盐有关的某些基因已转入小麦。

The seedlings of Agropyron elongatum ,wheat and their somatic hybrid lines Ⅱ 1,Ⅱ 2 were treated with different concentrations of NaCl.Their growth rates and NaCl tolerant index were measured and the contents of proline,Na +,K +,Ca 2+ were determined 5 days after treatment.The results showed that Agropyron elongatum had the characteristics of the monocotyledonous halophytes,while wheat was not NaCl tolerant.The harmless of salt began to appear at the concentration of 0.5% and increased quickly when the concentration raised.However the hybrids began to suffer at a higher concentration and the death rate was much smaller than wheat when the salt concentration reached to 1% and more.The measurement of growth rate,index number of NaCl tolerant and some physiological index also showed the hybrids possessed higher NaCl tolerant.According to the above result,it was proved that some genes relating to NaCl tolerant from Agropyron elongatum had been transferred into wheat.