海边香豌豆胚性愈伤组织的诱导和体细胞胚发生

Embryogenic callus induction and somatic embryo formation from hypocotyl explants of Lathyrus maritimus

将生长 1 4d的海边香豌豆 ( L athyrus maritimus( L.) Bigel)无菌苗下胚轴切成 0 .5cm左右的片段 ,置于含有 1 mg/L2 ,4- D,0 .5mg/L BA和 0 .5% Na Cl的 MS培养基中 ,2 8d后诱导出胚性愈伤组织。将其转入含有适当浓度 2 ,4- D的 MS培养基上 ,又 2 8d后可得到大量球形胚和心形胚以及极少量鱼雷胚和子叶胚。诱导体细胞胚适合的 2 ,4- D浓度为 0 .5mg/L。较高浓度的 2 ,4- D能阻止体细胞胚越过球形期发育 ,但有利于体细胞胚产率的提高。Na Cl对于胚性愈伤组织的诱导十分关键。体细胞胚发生的频率随胚性愈伤组织开始的几次继代降低 ,以后趋于稳定。

Within about 4 weeks after culturing the segments of hypocotyl of Lathyrus maritimus (L.) Bigel,embryogenic callus was induced on MS medium plus 1 mg/L 2,4 D、0.5 mg/L BA and 0.5% NaCl.Somatic embryos were obtained on 2,4 D supplemented MS medium.Optimum concentration of 2,4 D was 0.5 mg/L.Higher concentration of 2,4 D inhibited embryo development from passing heart shaped stage,yet enhanced embryo proliferation.NaCl had an important effect on the induction and maintenance of embryogenic callus.Before the adaption to salt stress was founded,frequency of somatic embryo formation declined with subculturing.No further development of somatic embryos was succeeded until cotyledonary stage.