Epstein-Barr病毒感染对CNE-2Z不同细胞组分中PKC和TPK活性的影响

THE EFFECT OF EPSTEIN-BARR VIRUS INFECTION ON THE ACTIVITIES OF PROTEIN KINASE C AND TYROSINE PROTEIN KINASE IN DIFFERENT SUBCELLULAR FRACTIONS OF CNE-2Z CELL LINE IN VITRO

研究EBV体外再感染CNE-2Z细胞后,不同组分中PKC(蛋白激酶C)和TPK(酪氨酸蛋白激酶)活性的影响,并探讨PKC和TPK活性与细胞增殖的关系。实验分三组即对照组、EBV组和EBV+TPA组,用免疫细胞化学(以小鼠抗EB病毒早期抗原)检测EBV在体外能否再感染CNE-2Z细胞,用特异底物法和特异激活剂法分别测定其PKC和TPK活性,MTT法检测CNE-2Z细胞体外增殖能力。结果显示未处理CNE-2Z细胞中PKC活性为膜性>胞 核>胞液,TPK为胞核>膜性>胞液。EBV和EBV+TPA再感染CNE-2Z细胞后,抑制细胞增殖,同时胞液PKC和TPK活性升高,膜性和胞核TPK和膜性PKC活性降低。本研究结果提示,EBV可能通过影响不同细胞组分中PKC和 TPK活性来调节CNE-2Z鼻咽癌细胞的增殖。

In order to explore the effect of EBV infection on the activities of protein kinase C(PKC)and tyrosine protein ki-nases (TPK)in different subcellular fractions of CNE-2Z cell line in vitro. Polymerase chain reaction (PCR) .immuno-cytochemical staining,MTT assay and kinase assay were employed in our experiment. The results showed that in vitro EBV can reinfect CNE-2Z cell line (positive rates of early antigen from group E and ET were markedly higher than of early antigen from group C. ). After reinfection by EBV and EBV combined with TPA,OD value of MTT of group E and group ET were distinctly lower than that of control group (P<0. 01). It was found that in group C the activity of PKC was membranous > nuclear >cytosolicj the activity of TPK was nuclear>membranous>cytosolic. After treatment with EBV and EBV combined with TPA.the membranous and nuclear TPK and membranous PKC were obviously decreased. The cytosolic PKC and TPK were significantly elevated. Our study suggested that the proliferation CNE-2Z cells infected by EBV and EBV combined with TPA is related to the changes of PKC and TPK activities in three different subcellular fractions in vitro.