白附子水提物抑制人骨髓神经母细胞瘤细胞增殖的实验研究

The experimental study of inhibition of effective constituent of Giant Typhonium Rhizome on human bone marrow neuroblastoma cell （SH-SY5Y） proliferation

目的探讨白附子水提物体外对肿瘤细胞增殖的抑制作用。方法以白附子水提物处理人骨髓神经母细胞瘤（SH—SY5Y）细胞24、48h后，应用细胞增殖检测试剂Kit-8（CCK罐）细胞增殖和毒性实验检测白附子水提物对SH—SY5Y细胞的增殖抑制作用，运用蛋白印记反应（AV／PI）对该细胞株生长抑制机理进行初步探讨。结果白附子水提物对SH．SY5Y细胞的增殖具有抑制作用，与细胞作用48h的半数抑制浓度（IC50）为0．862mg／mL，与细胞作用24h的IC50为1．158mg／ml。Western．blot法检测结果表明，白附子水提物可能通过抑制肿瘤细胞增殖、诱导凋亡抑制其生长。结论白附子水提物有抑制SH—SY5Y细胞增殖作用。

Objective To investigate the anti tumor effect of Giant Typhonium Rhizome. Methods SH-SY5Y was treated for 24 or 48 h by Giant Typhonium Rhizome. Cell Counting Kit-8 （CCK-8） cell proliferation and cytotoxicity assays were applied to detect the inhibition of SH-SY5Y cells treated with aqueous extract of white monkshood root. AV/PI double staining flow eytometry was applied to explore the mechanism of growth inhibition. Results SH-SY5Y cells could be inhibited by root aqueous extract of Giant Typhonium Rhizome, the IC50 at 48 h （50% inhibitory concentration） was 0.862 mg/ml, while the IC50 at 24 h was 1.158 mg/ml. Western-blot results showed that root aqueous extract of Giant Typhonium Rhizome could induce apoptosis to inhibit tumor cells. Conclusion The Rhizoma typhonii inhibit the growth of SH-SYSY.