五味降压方在药效反应时肠吸收多成分暴露特征探讨

Study on intestinal absorption of multiple constituents under the drug response of Wuwei-Jiangya recipe

目的研究五味降压方呈现降压反应时，大鼠肠吸收的多成分暴露特征。方法①五味降压方提取后灌胃给予大鼠，1次／d，直到产生降压反应；②采用翻转肠囊法，运用反相高效液相色谱指纹图谱技术，建立五味降压方以及肠吸收多成分暴露特征谱。结果五味降压方1周即可产生降压反应，有多种成分可被肠吸收。结论在药效反应发生时，采用翻转肠囊法研究肠吸收成分，可用于暴露成分的初步锁定。

Objective To research the intestinal absorption characteristics in rats of multiple constituents, when Wuwei-Jiangya recipe was used in rats and showed reducing blood pressure effects. Methods ① After extracting Wuwei-Jiangya recipe, we fed it to rats once daily, until the blood pressure reduced; ② Establish Wuwei-Jiangya recipe and intestinal absorption of multiple constituents fingerprint by using reverted gut sac method and RP-HPLC fingerprint. Results After one week＇s administration, there was the hypotensive effects and multiple constituents can be absorbed by intestine. Conclusion When the drug works, reverted gut sac method for studying intestinal absorption constituents can be used for locking the exposure constituents.